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Plasmid#78670PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_I13453 to adjust spacing in MoClo system. [A:36INS1-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
p416GAL1-Skn7t-L-IFP-F[1] (Yeast)
Plasmid#52896PurposeSkn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.DepositorInsertSkn7t-L-IFP-F[1]
UseTagsL-IFP-F[1]ExpressionYeastMutationPromoterGAL1Available sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYB2 reporter
Plasmid#197276PurposeEncodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseLentiviralTagsFLAGExpressionMutationN/APromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMy-BirA-T2A-eGFP
Plasmid#50565PurposeRetroviral vector encoding for GFP and the biotinylating enzyme BirADepositorInsertAmpicilin
UseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMy-CTCF-GFP
Plasmid#50574PurposeRetroviral vector endoding for mouse CTCF fused to GFPDepositorInsertAmpicilin
UseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBTK501
Plasmid#110602PurposeBTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)DepositorInsertGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pASPIre2
Plasmid#154843PurposeDerivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1DepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSNA
Plasmid#135192PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassetteDepositorInsertI-SceI lamba Red
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoteraraBADAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN41A-T38S38
Plasmid#49524PurposeAmpicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sitesDepositorInsertconstitutive TetR38
UseTagsExpressionMutationPromoterPtb38Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only