-
Plasmid#118738PurposeTo express membrane targeted GFPDepositorInsertLck domain (LCK Human)
UseLentiviralTagsGFPExpressionMutationH8N (see comments)PromoterCMVAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBBi-Pur GFP-TM
Plasmid#139185PurposeSB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.DepositorInsertGFP-TM
UseTransposonTagsExpressionMammalianMutationfused to membrane anchorPromoterEF1αAvailable sinceMarch 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-EGFP-CAAX
Plasmid#208683PurposeExpresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cellsDepositorInsertmembrane-localized enhanced green fluorescent protein EGFP-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT2 NCmut-msfGFP
Plasmid#180320Purposemammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFPDepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT2 F20D, V27DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_msfGFP-SEPT7 Gmut2
Plasmid#180425Purposemammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFPDepositorInsertSEPTIN7 (SEPTIN7 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT7 H279DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_msfGFP-SEPT7 Gmut1
Plasmid#180316Purposemammalian expression of human SEPT7 Gmut1 fused to monomeric superfolder GFPDepositorInsertSEPTIN7 (SEPTIN7 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT7 W269A, H279DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CXCR4-SZ158a-sfGFP
Plasmid#162446PurposeExpression in HEK293T cell and compete ligand singaling against full-length receptorsDepositorInsertC-X-C chemokine receptor type 4 (CXCR4 Human)
UseTagssfGFPExpressionMammalianMutationtruncation from aa52 to aa257PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-EGFP-GPI-stop-L2
Plasmid#186355PurposeEntry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequencesDepositorInsertsignal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site (CD59 Human, Synthetic)
UseExpression of a fluorescent membrane markerTagsEGFPExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GPI-mEGFP
Plasmid#182866PurposeMonomer expression vector with plasma membrane localization signalDepositorInsertGPI-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-Caveolin-C-10
Plasmid#56366PurposeLocalization: Membrane, Excitation: 485, Emission: 507DepositorInsertCaveolin (CAV1 Human)
UseTagssfGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits