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Plasmid#217960PurposeExpression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H4 (H4C1 Human)
UseTagseGFPExpressionMammalianMutationL63A, F66A, I71APromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1317
Plasmid#217959PurposeExpression of human H4 carrying a triple glycine insertion to disrupt folding with H3. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H4 (H4C1 Human)
UseTagseGFPExpressionMammalianMutationGGG insertion between V65 and F66PromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1314
Plasmid#217956PurposeExpression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFPExpressionMammalianMutationGGG insertion between A95 and C96PromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFX-H2B-Sirius
Plasmid#175837PurposeExpress Sirius with nucleus-targeting sequence in mammalian cellsDepositorInsertSirius with nucleus-targeting sequence
UseTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-H2A
Plasmid#36207PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorInsertH2A (H2AC16 Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-H2B FR-MQV
Plasmid#194079PurposeExpresses nuclear-localized FR-MQV in mammalian cellsDepositorInsertFR-MQV
UseTagsHistone H2BExpressionMammalianMutationMethionine 42 to Glutamine, Cystiene 159 to Valin…PromoterCMV7Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_H2A_C1
Plasmid#85051PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorInsertH2A (H2AC16 Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
iRFP-H2A
Plasmid#158691PurposeThis plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markersDepositorInsertiRFP670
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-Map4
Plasmid#105571PurposeT7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNADepositorInsertMAP4 (Map4 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationPromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+H1.4
Plasmid#157796PurposeExpression of human linker histone H1.4DepositorInsertH1.4 (H1-4 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-H2B-Dendra
Plasmid#140559PurposeRed to green photoconvertible chromosome markerDepositorInsertH2B
UseTagsDendraExpressionMammalianMutationPromoterCMV IE94Available sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
p55-H2B-Dendra2
Plasmid#80609PurposeEncodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.DepositorInsertH2B-Dendra2 (H2BC21 Synthetic, Human)
UseTagsDendra2ExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pL6-H2B-iRFP720-puro
Plasmid#189992PurposeLentivirial plasmid for nuclear iRFP-720 expression with puromycin resistanceDepositorInsertHuman histone H2B
UseLentiviralTagsiRFP720ExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp MCP-mCherry IRES h2b-diRFP
Plasmid#102351PurposeLentiral expression vector bearing the MS2 Coat Protein (MCP) fused to mCherry as well as the nuclear marker histone 2b fused to two copies of irFP. Also bears the hygromycin selection marker.DepositorInsertMS2 Coat Protein
UseLentiviralTagsmCherryExpressionMutationPromoterSFFVAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti.tie1.H2B.eYFP.W
Plasmid#51293PurposeLentivector that encodes H2B-eYFP under the transcriptional control of the mouse TIE1 promoterDepositorInsertH2B-eYFP (H2BC21 Human)
UseLentiviralTagsH2B-eYFPExpressionMutationPromotermTie1Available sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
Plasmid#40571DepositorInsertAurora B FRET-sensor N-terminally fused to histone 2B
UseTagsCyPet and YPetExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h1
Plasmid#86689PurposeFor transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'DepositorInserthistone-lysine N-methyltransferase KMT5B (Kmt5b Mouse)
UseTagsHA-tagExpressionMutationPromoterT3Available sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
ath5:H2B-RFP
Plasmid#105960Purposetransgenesis, appears rather late, likely due to long RFP maturation time but is brighter than most other Ath5 constructDepositorInsertH2B (H2BC21 Human)
UseTagsRFPExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti.hUbC.H2B-CeruleanFP-2A-Dendra2FP.W
Plasmid#126522PurposeLentivector that expresses H2B-Cerulean and Dendra2 fluorescent proteins from an internal hUbC promoter.DepositorInsertH2B-CeruleanFP-2A-Dendra2FP
UseLentiviralTagsExpressionMutationPromoterhUbCAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_018
Plasmid#123770PurposeEncodes human histone H2B as a Type 3a part to be used in the MTK systemDepositorInsertH2B
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only