We narrowed to 2,616 results for: pCas

Showing: 1061 - 1080 of 2616 results

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pX461-evoCDA1-nSpCas9-Intergenic sgRNA

Plasmid

#237464

Purpose

All-in-one base editor expressing cytosine base editor (evoCDA1-nSpCas9) and sgRNA control targeting Intergenic site

Depositor

Thomas Gonatopoulos-Pournatzis

Publication

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

Intergenic sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-ABE8e-nSpCas9-NRF1-ss-sgRNA

Plasmid

#237466

Purpose

All-in-one adenine base editor (ABE8e-nSpCas9) with gRNA targeting NRF1-Ex7 splice site

Depositor

Thomas Gonatopoulos-Pournatzis

Publication

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

gRNA targeting NRF1-Ex7 splice site

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-evoCDA1-nSpCas9-NRF1-ss-sgRNA

Plasmid

#237467

Purpose

All-in-one cytosine base editor (evoCDA1-nSpCas9) with sgRNA targeting NRF1-Ex7 splice site

Depositor

Thomas Gonatopoulos-Pournatzis

Publication

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

sgRNA targeting NRF1-Ex7 splice site

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pSPcas9(BB)-2A-Puro V2.0 sgCHMP2B-2 aauucccaaaugaagauggc

Plasmid

#231998

Purpose

Expression of an sgRNA targeting CHMP2B, Cas9, and a PuroR marker

Depositor

Franz-Ulrich Hartl

Publication

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B-targeted sgRNA (CHMP2B Human)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only
pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

Plasmid

#223111

Purpose

pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

Depositor

Benjamin Kleinstiver

Publication

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9-P2A-mTagBFP2

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-mTagBFP2

Expression

Mammalian

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-U6-BRD4-HaloTag-Guide-hspCas9

Plasmid

#228576

Purpose

Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

Depositor

Marcin Imielinski

Insert

BRD4 gRNA (BRD4 Human)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

Plasmid

#223323

Purpose

Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

Depositor

Jonathan Arias

Publication

Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

Insert

SpCas9 and sgRNA-T2 (cas9 )

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

Plasmid

#220494

Purpose

To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

Depositor

Sébastien Britton

Publication

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting LIG4 exon 3 (LIG4 Human)

Use

CRISPR

Promoter

U6

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJS-BCD-Strep-PCaspase-R153A

Plasmid

#214036

Purpose

Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceum

Depositor

Raymond Staals

Publication

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Tags

Strep-tag II

Expression

Bacterial

Mutation

R153A

Promoter

T7 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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PET-HypaR-SpCas9-NLS-6xHis

Plasmid

#207387

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-HiFi SpCas9-NLS-6xHis

Plasmid

#207378

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A, amino acids 1005-1013 replaced with two gl…

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PET-Blackjack SpCas9-NLS-6xHis

Plasmid

#207375

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Blackjack SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 1005-1013 replaced with two glycine

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCA23-sgRNA vector-U6-sgTS2 (SpCas9)

Plasmid

#199454

Purpose

U6-driven sgRNA targeting TS2 sequence (GGAGCTTACTGAGACTCTTC)

Depositor

Baohui Chen

Publication

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TS2 sgRNA

Use

CRISPR

Promoter

mouse U6

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCA28-pMa-PspCas13b crRNA-TS1

Plasmid

#199459

Purpose

U6-driven crRNA targeting TS1 sequence (CCTCCTCGGAGAGCATCGGTGC )

Depositor

Baohui Chen

Publication

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TS1 crRNA

Use

CRISPR

Promoter

mouse U6

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

Plasmid

#182126

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Publication

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Plasmid

#182121

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Publication

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-gRNA(SFTPCI73Tcorr)-SpCas9(BB)-2A-GFP

Plasmid

#187647

Purpose

Encodes gRNA to correct the SFTPC I73T mutation

Depositor

Darrell Kotton

Publication

Alysandratos et al Cell Rep. 2021 Aug 31;36(9):109636. doi:

Insert

I73T gRNA

Use

CRISPR

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

Plasmid

#161924

Purpose

To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

Depositor

Sébastien Britton

Publication

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

sgRNA targeting RDH11 exon 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_162 SpCas9 KES(1107-1109)GG

Plasmid

#179526

Purpose

For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Publication

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 KES(1107-1109)GG

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

replaced KES (residues 1107-1109) with GG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-3exo-Tetra-com-CLCN5-sp-g1

Plasmid

#176236

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Publication

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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