We narrowed to 5,650 results for: pCas

1. pCAG-Scarlet-Kif5a

   Plasmid

   #118745

   Purpose

   Expresses scarlet tagged Kif5a

   Depositor

   Beverly Koller

   Insert

   Kif5a cDNA (Kif5a Mouse)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-SRGAP2A-EGFP

   Plasmid

   #168501

   Purpose

   Expression of full length SRGAP2 with a C-termonal EGFP fusion

   Depositor

   Franck Polleux

   Article

   Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

   Insert

   SRGAP2A (SRGAP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAP03-sp44/p21

   Plasmid

   #120232

   Purpose

   Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

   Depositor

   Bradley Moore

   Article

   Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

   Insert

   bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   aac(3)IV (apramycin resistance gene)

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-HiBiT-CD81

   Plasmid

   #162593

   Purpose

   N-terminally HiBiT-tagged human CD81 under CAG promoter

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

   Insert

   human CD81 (CD81 human, Synthetic, Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG2-EGFP-C-IFT121

   Plasmid

   #218736

   Purpose

   Expresses C-terminally EGFP-tagged IFT121 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Insert

   IFT121 (WDR35 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AW01_pU.CAG.eSpCas9(1.1).rBGpA

   Plasmid

   #199255

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1) nuclease

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

   Insert

   High specificity eSpCas9(1.1) nuclease
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   K848A K1003A R1060A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HA-Nrxn1beta AS4(+)

   Plasmid

   #59410

   Purpose

   Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Nrxn1beta AS4(+) (Nrxn1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-SpCas9-HF1

   Plasmid

   #108301

   Purpose

   pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-memRFP-3xControlgRNA

   Plasmid

   #224567

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   CRISPR

   Tags

   Membrane localization signal

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG/ATP10D(E215Q)-HA

    Plasmid

    #209248

    Purpose

    Mammalian expression of ATP10D mutant E215Q

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10D (ATP10D Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E215Q

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG2-EGFP-C-IFT139

    Plasmid

    #218738

    Purpose

    Expresses C-terminally EGFP-tagged IFT139 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT139 (TTC21B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid

    #191442

    Purpose

    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hROSA26 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-ChrimsonR-mRuby2-ST

    Plasmid

    #105447

    Purpose

    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

    Insert

    ChrimsonR-mRuby2-ST
    

    Use

    Tags

    mRuby2 and soma targeting motif

    Expression

    Mammalian

    Mutation

    K176R in Chrimson

    Promoter

    pCAGGS

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCag FlpO-2A-Cre EV

    Plasmid

    #129419

    Purpose

    episomal expression of FlpO and Cre recombinases

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    FlpO-2A-Cre
    

    Use

    Cre/Lox and Unspecified; Episomal expression vect…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/Chick β-actin (CAG)

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG_Xph15-SNAPf-CCR5TC

    Plasmid

    #135536

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Use

    Tags

    SNAPf

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-5HA-mISG15

    Plasmid

    #12444

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    ISG15
    

    Use

    Tags

    5xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pCAGGS-FLAG-CaMKIIB

    Plasmid

    #186826

    Purpose

    Expresses FLAG-CaMKIIB in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2b (Camk2b Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-b3STOPb3-ZsGreen

    Plasmid

    #51266

    Purpose

    B3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    b3STOPb3-ZsGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-memRFP-3xPax7gRNA

    Plasmid

    #224569

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    CRISPR

    Tags

    Membrane localization signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG2-EGFP-C-IFT122

    Plasmid

    #218737

    Purpose

    Expresses C-terminally EGFP-tagged IFT122 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT122 (IFT122 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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