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pSMART-HCKan-yibDp-roGFP2

Plasmid

#202462

Purpose

Expresses redox sensitive GFP (roGFP) after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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Xlone Puro SNAP-eGFP-BASU

Plasmid

#218452

Purpose

expresses the SNAP-eGFP-BASU fusion protein via stable PiggyBac integration

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

SNAP-eGFP-BASU

Expression

Mammalian

Promoter

TRE3GS

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5_NLS-Halo-eGFP-BASU

Plasmid

#218449

Purpose

expressed the NLS-Halo-eGFP-BASU fusion protein via stable Flp-In integration

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

NLS-Halo-eGFP-BASU

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-BFP-P2A-ppViperin

Plasmid

#217481

Purpose

Expression of Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Article

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

BFP-P2A-otPKRFL

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pTSAR3.6t

Plasmid

#210258

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

mScarlet
superfolder GFP

Tags

ssra

Expression

Bacterial

Promoter

ipaH7.8p and rpsM

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTSAR3.7t

Plasmid

#210259

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

e2Crimson
superfolder GFP

Tags

ssra

Expression

Bacterial

Promoter

ipaH7.8p and rpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTSAR3.3t

Plasmid

#210255

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

DsRed
superfolder GFP

Tags

ssra

Expression

Bacterial

Promoter

ipaH7.8p and rpsM

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-BFP-P2A-otPKR-ML

Plasmid

#214365

Purpose

Expression of the Blue Fluorescent Protein - P2A - medium length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

BFP-P2A-otPKRML

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-BFP-P2A-otPKR-SL

Plasmid

#214366

Purpose

Expression of the Blue Fluorescent Protein - P2A - short length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

BFP-P2A-otPKRSL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-BFP-P2A-otPKR-FL

Plasmid

#214364

Purpose

Expression of the Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

BFP-P2A-otPKRFL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET28 bleach.2

Plasmid

#191878

Purpose

Encodes a GFP design, bleach.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.2

Tags

His Tag

Expression

Bacterial

Mutation

V61L T65S V68A S72A V112I Y145M

Promoter

T7

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 mid.8

Plasmid

#191902

Purpose

Encodes a GFP design, mid.8

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.8

Tags

His Tag

Expression

Bacterial

Mutation

V61A S72T Y145M L220V

Promoter

T7

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_CD-greenlantern

Plasmid

#202141

Purpose

Level 0 plasmid containing a green lantern fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Green lantern gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pFR-mTq2-antiFRET-P2A-RCaMP1h

Plasmid

#191467

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

RCaMP1h

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 sf:bleach.1

Plasmid

#191929

Purpose

Encodes a GFP design, sf:bleach.1

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:bleach.1

Tags

His Tag

Expression

Bacterial

Mutation

T108L Y145M T167V L220V

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.13

Plasmid

#191915

Purpose

Encodes a GFP design, mid.13

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.13

Tags

His Tag

Expression

Bacterial

Mutation

Q69L S72V Y145S

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.6

Plasmid

#191899

Purpose

Encodes a GFP design, mid.6

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.6

Tags

His Tag

Expression

Bacterial

Mutation

V68M S72T Y145M V224I

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.5

Plasmid

#191896

Purpose

Encodes a GFP design, mid.5

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.5

Tags

His Tag

Expression

Bacterial

Mutation

Y145M T167V H181Y L220V V224I

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.4

Plasmid

#191895

Purpose

Encodes a GFP design, mid.4

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.4

Tags

His Tag

Expression

Bacterial

Mutation

V68A S72A Y145M L220V

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-mCherry

Plasmid

#191466

Purpose

Compare the intensity of mCherry to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

mCherry

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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