...resource investment required to change the status quo. But with recent improvements to ELN and online lab...

... B. et al. Open microscopy in the life sciences: quo vadis?. Nat Methods 19, 1020–1025 (2022). https:/...

...36201 pLifeAct-mTurquoise2 Actin Filaments LifeAct mTurquoise2 Dorus Gadella 36202 pmTurquoise2-Tubulin Microtubules...Gadella 137803 EMTB-mTurquoise2 Microtubules EMTB mTurquoise2 Dorus Gadella 137807 mTurquoise2-MapTau Microtubules...mNeonGreen Dorus Gadella 98817 3xnls-mTurquoise2 Nucleus 3xNLS mTurquoise2 Dorus Gadella 98816 3xnls-mScarlet-I...mNeonGreen Dorus Gadella 98819 4xmt-mTurquoise2 Mitochondria 4xmts mTurquoise2 Dorus Gadella 98818 4xmts-mScarlet-I...Vladislav Verkhusha 36208 pmTurquoise2-Mito Mitochondria COX8A(1-29) mTurquoise2 Dorus Gadella 26673 pEGFP-C1...Fibrillarin EGFP Sui Huang 36207 pmTurquoise2-H2A Nucleus H2A mTurquoise2 Dorus Gadella 85053 pmScarlet-...mScarlet-I Dorus Gadella 98830 mTurquoise2-LaminB Nuclear Envelope LaminB mTurquoise2 Dorus Gadella 55069 mCherry-LaminB1...

...a similarly-sized aliquot from an amplification done by Addgene.) This small aliquot is not enough to ... lentiviral generation of a pooled library. An aliquot of the pooled library is transformed into E. coli...Pooled library lentiviral preps are generally aliquoted to reduce the number of freeze-thaw cycles needed...needed when using the virus. A small aliquot is used to determine functional titer with a colony formation...library from Addgene, you’ll usually receive an aliquot from the original library constructed by the depositing...sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests specifically to allow...type, and other factors, so make sure to put that aliquot to full use! Common conditions to optimize include...

...of monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2 to various subcellular...pmVenus(L68V)-mTurquoise2 Brightness standard used as positive control to characterize mTurquoise2 pmTurquoise2...negative control (no FRET) with pmVenus(L68V)-mTurquoise2 Back to Top Do you have suggestions for other...

...N86/38R-2b] GFP Aequorea victoria Mouse IgG2b 199420 Anti-GFP [N86/8R-2b] GFP Aequorea victoria Mouse ...[N86/38R-1] GFP Aequorea victoria Mouse IgG2a 206720 Anti-GFP [N86/8R-1] GFP Aequorea victoria Mouse IgG1...N86/44 scFv GFP Aequorea victoria Mouse 206744 GFP scFv [N86/20] N86/20 scFv GFP Aequorea victoria Mouse...Rat Mouse IgG2a 114492 Anti-GFP [N86/38.1R] GFP Aequorea victoria Mouse IgG2a 114493 Anti-NGL-3/LRRC4B ...receptor Rat Mouse IgG2a 177572 Anti-GFP [N86/8R] GFP Aequorea victoria Mouse IgG2a 177573 Anti-GABA(A)R, Beta3...VAPA/B Mouse IgG2a 188164 Anti-GFP [N86/44R] GFP Aequorea victoria Mouse IgG2a 188165 Anti-Prrt2 [N485/22R...Mouse Mouse IgG2a 188166 Anti-GFP [N86/20R] GFP Aequorea victoria Mouse IgG2a 188167 Anti-IP3 receptor,...

...non-aggregating fluorescent protein. In the jellyfish Aequorea victoria, AvGFP forms a homodimer. In corals, ...OSER assay including CytERM-mKOkappa, CytERM-mTurquoise2, and CytERM-dTomato. When using and interpreting...fluorescent proteins. Fusions of CytERM with mTurquoise2 and dTomato, which are established monomeric ...of new fusions can be compared with mEGFP or mTurquoise2, which are established true monomeric fluorescent...

...same plate) with purified plasmid from the same aliquot/preparation. The replicate control isn’t measuring...replicate should use fresh media, test independent aliquots of cells and plasmids, be performed on different...different preparations of Plasmid A in different aliquots of cells on several different days. Biological...phenotype isn’t singular or associated with only one aliquot/preparation of the test plasmid.  Let us now revisit...

...matters The green fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded...behave as monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen, mScarlet(-I), and mCherry...-terminus of fluorescent proteins derived from Aequorea victoria GFP sticks out and can be considered ...from N- to C-terminus. For instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid...

... 10 × 100 µL aliquots 1 mL 4 × 10 12 GC/mL* 1 × 10 13 GC/mL* Medium 25 × 100 µL aliquots 2.5 mL Large ...Large 50 × 100 µL aliquots 5 mL *AAV2 yields are generally lower, with a minimum titer of 2 × 10 12 GC/mL ...

... When aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots to use...helps avoid multiple freeze/thaws of your larger aliquots of virus. AAV titration by qPCR Overview: AAV...

...erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein Sensor...13(5):521-30. Amy Palmer , Roger Tsien Calcium Turquoise genetically encoded calcium sensor (GECI) that...reports with lifetime and intensity changes A turquoise fluorescence lifetime-based biosensor for quantitative...

...efficacy of our antibodies through stress testing. An aliquot of our recombinant antibodies is stored at 37 °... are still equally effective compared to those aliquots frozen immediately post-harvest. Stress-tested...shifts. Of course, we are also cautious during the aliquoting and storage process to prevent dropped tubes....

...adequate for in vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer Titering is either...polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, and stored at -80 °C. Concentrated lentiviral... viral particles are then resuspended in PBS, aliquoted, frozen, and stored at -80 °C. Titer All titering...

...monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2 to various subcellular...mCerulean/mVenus, mCerulean/Amber, mCerulean/SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the...

...yourself.  Fig 1: Quote from Michael Ende, courtesy of morefamousquotes.com A good listener absorbs...maintaining their friendships. I put “neutral” in quotations here because I would not have considered myself...

...Expression mTurquoise2 434 474 28 3.1 Monomer (A206K) pmTurquoise2-C1 - Mammalian Expression pmTurquoise2-N1 ...

... Cap subject industry H-1B There is a numerical quota of 85,000 total H-1B visas approved each fiscal ...organizations do not have the April 1 H-1B queue process or quota like industry, these organizations can be a good...allows employers to avoid the 85,000-visa numerical quota. Four types of employers are not subject to the ...

...adequate for in vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer Titering is either...polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, and stored at -80 °C. Titer All titering...

...note that all orders will be shipped in 100 µL aliquots, so the volume options will appear as 10, 25, ...AAVrg and 7 × 1012 GC/mL for AAV2. There are no quotes or special processes involved; simply request your...