... through a 0.22 µm filter. Aliquot 50–100 µL and store at -20 °C. Aliquots can be thawed and stored at...at 4 °C prior to use. Thawed aliquots should be discarded after 1–2 months. 1 mg/mL polyethylenimine, ...Filter the solution through a 0.22 µm membrane. Aliquot 500–1000 µL into sterile tubes. Store the tubes...

...1.0 μg/μL = 468.1 μL Aliquot 100 mL of OptiMEM into a sterile 250 mL bottle. Aliquot 774 mL of DMEM + 2%...Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into sterile tubes. Store the tubes...recommended or the solution may separate into phases. Aliquot and store at 4 °C. Cell Lysis Buffer : 50 mM Tris...

... 200 µL micropipette tips in a -20 °C freezer. Aliquot 3 mL SOC into each of four 14 mL Vented Falcon .... Procedure Day 1 Add 200 ng DNA to each 50 µL aliquot of thawed Endura Duos on ice. Flick gently to mix...Micropulser Ec1 0.1 cm cuvette, 1.8 kV, 1 pulse. Aliquot 25 µL DNA-Endura into pre-chilled cuvette. Pulse...containing 3 mL SOC. Repeat for each of the 25 µl aliquots of cell/DNA mixture used (8 in the described protocol...

... complete. Mix well by pipetting or inverting. Aliquot 2 mL of cell suspension into each well of the 6...cycles of freeze-thaw. If you are freezing and aliquoting virus, it is recommended that you titer from ...using frozen virus, rapidly thaw the lentiviral aliquot on ice prior to use. Prepare dilutions of the lentivirus...

...cycles of freeze-thaw. If you are freezing and aliquoting virus, it is recommended that you titer from ...freeze-thaw. If using frozen virus, thaw the lentiviral aliquot on ice prior to use. Prepare 10-fold serial dilutions...polybrene. Mix well by pipetting or inverting. Aliquot 1.35 mL of the cell suspension into each well of...

...through a sterile 0.22 µm filter. Prepare 10 mL aliquots and store the solution at -20 °C. 1 mg/mL PEI-...the solution through a 0.22 µm filter. Prepare aliquots and store at -20 °C until use. BCD TFX 1000 mL...sterilize through a 0.2 µm PES syringe filter. Aliquot and freeze upright at -20 °C. Procedure Section...

...Electrophoresis chamber Pipetman Reagents Liquid DNA aliquot of your plasmid of interest (see below for recommend...of the reaction components except for the DNA. Aliquot your DNA into individual tubes and then add the...

...Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into sterile tubes. Store the tubes...stored at 4 °C for several hours but should be aliquoted and snap frozen in liquid nitrogen and stored ...

...polybrene into 20 mL media. Thaw the lentiviral aliquot on ice prior to use. Prepare a range of dilutions.... Mix well by pipetting or inverting the tube. Aliquot 1 mL of cell suspension (i.e., 50,000 cells) into...

...well and sterilize through a 0.2 µm PES filter. Aliquot and freeze upright at -20 °C Procedure Section ...Centrifuge at 3100 x g for 8 min . Remove a small aliquot of your sample from the filter reservoir to check...

...contaminating reagents we recommend making small aliquots of master mixes, primers, and probes prior to ...gently uncap the tubes. Add 5 µL of the previously aliquoted 1X dilution buffer from step 9 to the NTC tube...

...your entire RNA sample, consider making smaller aliquots of it and storing those in -80°C. Option #2 - ...your entire RNA sample, consider making smaller aliquots of it and storing those in -80°C. Solution D Protocol...

...recommends that you use the virus immediately or aliquot the media into smaller tubes to prevent multiple...Sterilize by passing through a 0.22 μm filter. Store aliquots at -20°C. I.3. Warranty Information Addgene is...

...chamber Pipetman Pipet tips Reagents Liquid DNA aliquot of your plasmid of interest (see below for recommended...

...idea to take a fresh tube, thaw it one time and aliquot individual tubes of 5, 10 or 20μL for storage at...

...possible. Store at 4 °C for short term (2 weeks), or aliquot and store at -80 °C for long term. Sample Data ...

...culture cannot fit into a single centrifuge tube, aliquot it into several tubes/bottles. Remove the supernatant...

...validated standard curve is obtained, make a small aliquot of each standard (enough for 1 or 2 plates) and...

...contaminating reagents we recommend making small aliquots of master mixes, and primers/probe mixes prior...