...resource investment required to change the status quo. But with recent improvements to ELN and online lab...

... B. et al. Open microscopy in the life sciences: quo vadis?. Nat Methods 19, 1020–1025 (2022). https:/...

...a similarly-sized aliquot from an amplification done by Addgene.) This small aliquot is not enough to ... lentiviral generation of a pooled library. An aliquot of the pooled library is transformed into E. coli...Pooled library lentiviral preps are generally aliquoted to reduce the number of freeze-thaw cycles needed...needed when using the virus. A small aliquot is used to determine functional titer with a colony formation...library from Addgene, you’ll usually receive an aliquot from the original library constructed by the depositing...sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests specifically to allow...type, and other factors, so make sure to put that aliquot to full use! Common conditions to optimize include...

...non-aggregating fluorescent protein. In the jellyfish Aequorea victoria, AvGFP forms a homodimer. In corals, ...OSER assay including CytERM-mKOkappa, CytERM-mTurquoise2, and CytERM-dTomato. When using and interpreting...fluorescent proteins. Fusions of CytERM with mTurquoise2 and dTomato, which are established monomeric ...of new fusions can be compared with mEGFP or mTurquoise2, which are established true monomeric fluorescent...

...same plate) with purified plasmid from the same aliquot/preparation. The replicate control isn’t measuring...replicate should use fresh media, test independent aliquots of cells and plasmids, be performed on different...different preparations of Plasmid A in different aliquots of cells on several different days. Biological...phenotype isn’t singular or associated with only one aliquot/preparation of the test plasmid.  Let us now revisit...

...matters The green fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded...behave as monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen, mScarlet(-I), and mCherry...-terminus of fluorescent proteins derived from Aequorea victoria GFP sticks out and can be considered ...from N- to C-terminus. For instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid...

... When aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots to use...helps avoid multiple freeze/thaws of your larger aliquots of virus. AAV titration by qPCR Overview: AAV...

...efficacy of our antibodies through stress testing. An aliquot of our recombinant antibodies is stored at 37 °... are still equally effective compared to those aliquots frozen immediately post-harvest. Stress-tested...shifts. Of course, we are also cautious during the aliquoting and storage process to prevent dropped tubes....

...adequate for in vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer Titering is either...polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, and stored at -80 °C. Concentrated lentiviral... viral particles are then resuspended in PBS, aliquoted, frozen, and stored at -80 °C. Titer All titering...

...monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2 to various subcellular...mCerulean/mVenus, mCerulean/Amber, mCerulean/SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the...

...yourself.  Fig 1: Quote from Michael Ende, courtesy of morefamousquotes.com A good listener absorbs...maintaining their friendships. I put “neutral” in quotations here because I would not have considered myself...

... Cap subject industry H-1B There is a numerical quota of 85,000 total H-1B visas approved each fiscal ...organizations do not have the April 1 H-1B queue process or quota like industry, these organizations can be a good...allows employers to avoid the 85,000-visa numerical quota. Four types of employers are not subject to the ...

...note that all orders will be shipped in 100 µL aliquots, so the volume options will appear as 10, 25, ...AAVrg and 7 × 1012 GC/mL for AAV2. There are no quotes or special processes involved; simply request your...

...department requires a quote in order to generate a PO, learn more about how to create a quote. How do I pay ...

...bioluminescent properties observed in the crystal jellyfish Aequorea victoria (Figure 1) in molecular detail. On the...jellyfish’s photoorgans; the first, which he called aequorin, gives off a faint blue light when it binds calcium...

...molecular biologists bought the pricey stuff in 50 ul aliquots in a kit with a huge amount of wasteful packaging...from a pottery supply house.  These large, cheap aliquots could be stored indefinitely at room temperature...

...going through small aliquots at a time, the -20°C is your best option, in aliquots no smaller than 10uL...

... any project in which you need to do a lot of aliquoting. Simply program the pipette with how much total... sample you need and the volume of the smaller aliquots you’d like to dispense and you’re on your way....

...genetically encoded fluorescent markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list...service By Ina Ersing We regularly add new viral aliquots from our plasmid collection to provide ready-to-use...

...conditions, with pKa values below 4. Examples include mTurquoise2 (pKa = 3.1), tagRFP (pKa = 3.1) and mCherry (...pKa = 3.8). We have observed that mCherry (and mTurquoise2, not shown) remains fluorescent in acidic organelles...