We narrowed to 15,772 results for: LEA

1. pLenti-HIFR

   Plasmid

   #192946

   Purpose

   Lentiviral production of HIF-1alpha reporter

   Depositor

   Markus Covert

   Article

   Jeknic et al J Biol Chem. 2023 Mar 10:104599. doi: 10

   Insert

   HIF-1alpha reporter
   

   Use

   Lentiviral

   Promoter

   mCMV

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST14-DogCatcher

   Plasmid

   #171772

   Purpose

   Expresses DogCatcher protein in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   DogCatcher
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. RpHLuorin2

   Plasmid

   #180227

   Purpose

   for bacterial expression of recombinant ratiometric pHluorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2
   

   Tags

   His-tag followed by thrombin cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETNb-cALFA

   Plasmid

   #159989

   Purpose

   For the protein expression with ALFA tag at C-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Bacterial

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. H2B-HT-emiRFP703

   Plasmid

   #183985

   Purpose

   Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

   Depositor

   Pablo Rivera-Fuentes

   Article

   Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

   Insert

   H2B (H2BC11 Human)
   

   Tags

   HaloTag, emiRFP703

   Expression

   Mammalian

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTH2

   Plasmid

   #84452

   Purpose

   shuttle plasmid for sarA P1-CFP expression

   Depositor

   Reindert Nijland

   Article

   de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

   Insert

   CFP Cerulean
   

   Use

   Shuttle vector e.coli-s.aureus, expression of sgf…

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET302-6His-dCas9-Halo

   Plasmid

   #72269

   Purpose

   Encodes a Halo-labeled fusion of nuclease deficient Cas9

   Depositor

   Timothée Lionnet

   Article

   Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

   Insert

   His6-dCas9-Halo
   

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ404-DogCatcher-sfGFP

   Plasmid

   #171930

   Purpose

   Express DogCatcher-superfolder GFP protein in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   DogCatcher-sfGFP
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE2_CMV_Mex3a_t2a_mCherry

   Plasmid

   #223244

   Purpose

   tetO responsive expression of Mex3a in mammalian cells with t2a cleaved mCherry

   Depositor

   Stavros Lomvardas

   Insert

   Mex3a (Mex3a Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   amino acids conserved, underlying DNA sequence re…

   Promoter

   CMV

   Available Since

   May 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Prab-3::NLS::GFP

    Plasmid

    #68120

    Purpose

    Nuclear Localized GFP for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    GFP
    

    Expression

    Worm

    Promoter

    rab-3

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

    Plasmid

    #175521

    Purpose

    Nuclear expression of HaloTag10 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10
    

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-AktAR2

    Plasmid

    #64932

    Purpose

    Enhanced FRET-based biosensor for monitoring Akt activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    AktAR2
    

    Tags

    Cerulean3 and cpVenus[E172]

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNCS YFP

    Plasmid

    #91759

    Purpose

    Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

    Insert

    Venus YFP (A206K)
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

    Plasmid

    #175544

    Purpose

    Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag9-myc_PDGFRtmb
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PKCalpha.FLAG

    Plasmid

    #10805

    Depositor

    Alex Toker

    Article

    Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

    Insert

    PKC alpha (PRKCA Bovine)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D332G-please see comments below

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. MLM3613

    Plasmid

    #42251

    Purpose

    Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    CRISPR; Zebrafish expression

    Promoter

    CMV

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

    Plasmid

    #175522

    Purpose

    Nuclear expression of HaloTag11 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag11
    

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTP1005

    Plasmid

    #104160

    Purpose

    Bacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-syn-ChR2-EYFP-Kv

    Plasmid

    #89256

    Purpose

    Channelrhodopsin localized to neuronal soma via a Kv2.1 targeting motif

    Depositor

    McLean Bolton

    Article

    Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

    Insert

    Channelrhodopsin
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    synapsin

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-OSF-PP

    Plasmid

    #154247

    Purpose

    backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Type

    Empty backbone

    Tags

    FLAG and Strep-Tag II

    Expression

    Mammalian

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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