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pUBC(k)-AT5G01640-mKOk

Plasmid

#224847

Purpose

Plasmid for expression of AT5G01640.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT5G01640.1 (PRA1.B5 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-delVP2-VP2-454TAG

Plasmid

#240060

Purpose

Express AAV2 capsid with ncAA mutant at site 454 of VP2

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Inserts

RC2-DelVP2
VP2-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-delVP1-VP1-454TAG

Plasmid

#240059

Purpose

Express AAV2 capsid with ncAA mutant at site 454 of VP1

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Inserts

RC2-DelVP1
VP1-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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T7pro-tRNA-ITSIle intron-T7tVar1-pRS426

Plasmid

#239294

Purpose

Expresses the tRNAIle intron bearing the 5' GGGAGA initially transcribed sequence (ITS); Plasmid #2 of the tet-on overexpression system

Depositor

Anita Hopper

Article

Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

Inserts

T7 promoter
ITS-tRNAIle intron
T7 terminator - Var1

Expression

Yeast

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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LLPS-pCDH-DDX6-CT

Plasmid

#240014

Purpose

Expresses human DDX6-CT fused to EGFP in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-CT (303-483)

Use

Lentiviral

Tags

6xHis and EGFP

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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LLPS-pCDH-DDX6-ΔDEAD

Plasmid

#240017

Purpose

Expresses human DDX6-ΔDEAD fused to EGFP in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-ΔDEAD

Use

Lentiviral

Tags

6xHis and EGFP

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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LLPS-pCDH-DDX6-ΔIDR

Plasmid

#240016

Purpose

Expresses human DDX6-ΔIDR fused to EGFP in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-ΔIDR

Use

Lentiviral

Tags

6xHis and EGFP

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-Mouse-sgDdx6-1

Plasmid

#240003

Purpose

Expresses Mouse sgDdx6 in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

sgDdx6

Use

Lentiviral

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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LLPS-pCDH-DDX6-NT

Plasmid

#240015

Purpose

Expresses human DDX6-NT fused to EGFP in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-NT (1-302)

Use

Lentiviral

Tags

6xHis and EGFP

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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IMPT-11371

Plasmid

#236191

Purpose

Express GPR119 in insect cells

Depositor

Vadim Cherezov

Article

Kim et al Structure. 2024 Dec 5;32(12):2206-2219.e

Insert

GPR119 (GPR119 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-Halo-NLS-tDeg

Plasmid

#236370

Purpose

Overexpression of Halo-NLS-tDeg in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

Halo-NLS-tDeg

Use

Lentiviral

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA595 - pBA904 Puro-T2A-mCherry

Plasmid

#238171

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

mCherry

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA594 - pBA904 Puro-T2A-BFP

Plasmid

#238170

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK2(delta MTS)-3xFLAG

Plasmid

#233896

Purpose

lentiviral overexpression vector for NADK2 (delta MTS)

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

NADK2 (NADK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

delta MTS (see Depositor Comments)

Promoter

Ubc

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgNADK2

Plasmid

#233897

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting NADK2

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

sgRNA targeting NADK2 (NADK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pM-mCardinal

Plasmid

#228630

Purpose

Mammalian expression of mCardinal and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pM-mPapaya

Plasmid

#228626

Purpose

Mammalian expression of mPapaya and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pQdCas9-sggfp

Plasmid

#236184

Purpose

The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

Quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene

Promoter

Quorum sensing promoter

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pQdCas12a.sggfp(A)

Plasmid

#236038

Purpose

The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pQdCas12a.sggfp(B)

Plasmid

#236040

Purpose

The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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