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pDONR223_LNX1_p.V564I

Plasmid

#81521

Purpose

Gateway Donor vector containing LNX1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

LNX1 (LNX1 Human)

Use

Gateway entry vector

Mutation

V564I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ADORA2A_p.R220W

Plasmid

#81425

Purpose

Gateway Donor vector containing ADORA2A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ADORA2A (ADORA2A Human)

Use

Gateway entry vector

Mutation

R220W

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DNASE2_p.Y182H

Plasmid

#81374

Purpose

Gateway Donor vector containing DNASE2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DNASE2 (DNASE2 Human)

Use

Gateway entry vector

Mutation

Y182H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXO15_p.E410K

Plasmid

#81405

Purpose

Gateway Donor vector containing FBXO15 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXO15 (FBXO15 Human)

Use

Gateway entry vector

Mutation

E410K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTCF_p.N568K

Plasmid

#81612

Purpose

Gateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTCF (CTCF Human)

Use

Gateway entry vector

Mutation

N568K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARAF_WT

Plasmid

#82096

Purpose

Gateway Donor vector containing ARAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ARAF (ARAF Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_A4GALT_WT

Plasmid

#81835

Purpose

Gateway Donor vector containing A4GALT , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

A4GALT (A4GALT Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ING1_WT

Plasmid

#82213

Purpose

Gateway Donor vector containing ING1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ING1 (ING1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ASCL4_WT

Plasmid

#81909

Purpose

Gateway Donor vector containing ASCL4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ASCL4 (ASCL4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDKN1B_p.C29F

Plasmid

#81307

Purpose

Gateway Donor vector containing CDKN1B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDKN1B (CDKN1B Human)

Use

Gateway entry vector

Mutation

C29F, V109G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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piggyBac_Tet-On_HsZSWIM8(H395E, H401E, R404E, K460E, R461E, H464E, K465E, K466E, R470E, K808E, K815E, K817E, K818E, K820E,R825E)

Plasmid

#254372

Purpose

Expression of ZSWIM8 to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human ZSWIM8 (KIAA0913 Human)

Expression

Mammalian

Mutation

H395E, H401E, R404E, K460E, R461E, H464E, K465E, …

Promoter

TRE3G

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac CAG eGFP-PolQ-FLAG P2A-BLAST

Plasmid

#249811

Purpose

Expresses the PolQ (polymerase theta) protein fused with N-terminal eGFP and C-terminal FLAG tag

Depositor

Raphael Ceccaldi

Article

Gelot et al Nature. 2023 Sep;621(7978):415-422. doi:

Insert

Polymerase Theta (POLQ Human)

Tags

FLAG and eGFP

Expression

Mammalian

Promoter

CAG

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2729 - pLenti-X1-EF1A-HA-FBXO31(∆F-box, D334N)-PGK-Neo

Plasmid

#250261

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box, D334N)).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

∆F-box, D334N

Promoter

EF-1a

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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LentiX-(loxPcon-TET-DIAL-YB-TATA)-mCherry-HRasG12V-bGH-EFS-rtTA-TagBFP-WPRE

Plasmid

#246367

Purpose

loxPcon TET-DIAL Reporter Lentivirus with YB_TATA expressing mCherry-HRasG12V in the presence of DOX and rtTA and editable by Cre recombinase. Contains rtTA-TagBFP expressed divergenty.

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mCherry-GS-HRasG12V (HRAS Human)

Use

Lentiviral and Synthetic Biology

Mutation

G12V

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2735 - pHR-EF1A-HA-FBXO31(∆F-box, D334N)-IRES-GFP

Plasmid

#250267

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box, D334N)).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

∆F-box, D334N

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2733 - pHR-EF1A-HA-FBXO31(∆F-box, T343V)-IRES-GFP

Plasmid

#250265

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box, T343V)).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

∆F-box, T343V

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2738 - pHR-EF1A-DD-3xFLAG-FBXO31(D334N)-IRES-GFP

Plasmid

#250269

Purpose

Inducible expression of FBXO31(D334N) with the shield degron system.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

DD-3xFLAG

Expression

Mammalian

Mutation

D334N

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLCKO-CMV-cMyc-UBC-IRES-Blasti

Plasmid

#242462

Purpose

Expresses human UBC protein with an N-terminal cMyc tag enabling UBC pull down along with a blasticidin selection marker.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

UBC (UBC Human)

Use

Lentiviral

Tags

cMyc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-puro-EF1A-3XFLAG-hASB8_G198R

Plasmid

#242465

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The G198R mutation disrupts XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

G198R

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-puro-EF1A-3XFLAG-hASB8_L199R

Plasmid

#242466

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The L199R mutation disrupts XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

L199R

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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