We narrowed to 15,772 results for: LEA

1. pHB-4

   Plasmid

   #140957

   Purpose

   For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

   Depositor

   Kang Zhou

   Article

   Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

   Insert

   phaCAB
   

   Expression

   Bacterial

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. UAS-Syt::7xHalo7

   Plasmid

   #87650

   Purpose

   For creating drosophila transgenics expressing UAS-Syt::7xHalo7

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   Syt::7xHalo7
   

   Expression

   Insect

   Mutation

   S265G in Syt (please see depositor's commen…

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-iGlu3Fast

   Plasmid

   #226500

   Purpose

   Ultrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate release

   Depositor

   Katalin Torok

   Article

   Tran et al bioRxiv, August 30th, 2024

   Insert

   iGluSnFR3 S72T
   

   Tags

   IgK, Myc tag, and PDGFR Beta TM domain

   Expression

   Mammalian

   Mutation

   S72T

   Promoter

   CMV

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbAW4k-loxP-TT-loxP-mRFP1

   Plasmid

   #134405

   Purpose

   Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

   Depositor

   Mary Dunlop

   Article

   Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

   Insert

   W4-loxP-TT-loxP-mRFP1
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Bacterial

   Promoter

   W4 (modified T7A1)

   Available Since

   Jan. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE30-His-RF3

   Plasmid

   #124131

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Release factor 3 (prfC Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAM5505

   Plasmid

   #132664

   Purpose

   pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Insert

   MobA
   

   Expression

   Bacterial

   Mutation

   please see depositor comments below

   Promoter

   p1,p3

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b-His-RF2

   Plasmid

   #124130

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Release factor 2 (prfB Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK792

   Plasmid

   #8830

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   TEV protease, S219P mutant
   

   Tags

   His, MBP (cleaved), and polyarginine

   Expression

   Bacterial

   Mutation

   S219P mutation

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJExpress414_GmPOPB-S577A

   Plasmid

   #92235

   Purpose

   Express S577A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-S577A mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Serine 577 to Alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJExpress414_GmPOPB-D661A

    Plasmid

    #92236

    Purpose

    Express D661A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-D661A mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Aspartate 661 to Alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJExpress414_GmPOPB-H698N

    Plasmid

    #92242

    Purpose

    Express H698N mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-H698N mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Histidine 698 to Asparagine

    Promoter

    T7 promoter

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMJ920

    Plasmid

    #42234

    Depositor

    Jennifer Doudna

    Article

    Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    GFP, HA epitope, and NLS (nuclear localization si…

    Expression

    Mammalian

    Mutation

    codon-optimized synthetic DNA sequence

    Promoter

    CMV

    Available Since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. PARG-c002

    Plasmid

    #173107

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARG (PARG Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    S448-T976

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. SBE-NLS-mCherry-P2A-CreERT2 PGK-rtTA3

    Plasmid

    #196936

    Purpose

    TGFbeta reporter driven nuclear mCherry and CreERT2 with PGK driven rtTA3

    Depositor

    Elaine Fuchs

    Article

    Oshimori et al Cell. 2015 Feb 26;160(5):963-976. doi: 1

    Insert

    mCherry
    

    Use

    Lentiviral and RNAi

    Tags

    P2A-CreERT2

    Expression

    Mammalian

    Promoter

    SBE

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-OTI-TCRA_p2_TCRB

    Plasmid

    #131033

    Purpose

    Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Inserts

    TCRA (Tcra Mouse)
    TCRB (Tcrb Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV/T7

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLBac-Bat-FluA-trimer

    Plasmid

    #229858

    Purpose

    Influenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)

    Depositor

    Stephen Cusack

    Article

    Pflug et al Nature. 2014 Dec 18;516(7531):355-60. do

    Inserts

    PA_Bat-FluA_AFC35437.1
    PB1_Bat-FluA_AFC35436
    PB2_Bat-FluA_AFC35435.1
    

    Expression

    Insect

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST14-DoubleCatcher

    Plasmid

    #216284

    Purpose

    Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWT35S-mCherry-NLS

    Plasmid

    #194049

    Purpose

    Transient expression of mCherry-NLS in plant cell (Nucleus)

    Depositor

    Yutaka Kodama

    Article

    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

    Insert

    mCherry
    

    Tags

    SV40 (Nuclear localization signal)

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHB-3

    Plasmid

    #140956

    Purpose

    For PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Expression

    Bacterial

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSCW01

    Plasmid

    #72300

    Purpose

    Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Type

    Empty backbone

    Use

    Derivative of puc19

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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