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pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)

Plasmid

#110558

Purpose

miniTn7 delivery plasmid with lacIq-Ptac inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

lacIq-Ptac

Expression

Bacterial

Promoter

lacIq-Ptac

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Ai66(RCRL-tdT) targeting vector

Plasmid

#61578

Purpose

Target a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

CAG-RSR-LSL-tdTomato

Use

Cre/Lox and Mouse Targeting

Promoter

CAG

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Lin28

Plasmid

#60345

Purpose

Mammalian Expression, Lentiviral

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Lin28 (Lin28a Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pGFP FRNK

Plasmid

#50518

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

Insert

protein tyrosine kinase, mutated

Tags

GFP

Expression

Mammalian

Mutation

truncated (NT2186-3268)

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pBAD/HisB-iRFP702

Plasmid

#45455

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP702

Expression

Bacterial

Mutation

T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-ARAP3

Plasmid

#39484

Depositor

Sonja Vermeren

Article

Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

Insert

ARAP3 (ARAP3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
mEgr1/LZRS

Plasmid

#27783

Depositor

David Wiest

Insert

Egr1 (Egr1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
Actin-53a-ires-EGFP

Plasmid

#25460

Depositor

Jerry Crabtree

Article

Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

Insert

BAF53a (Actl6a Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 14, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PHD1

Plasmid

#21400

Depositor

Eric Metzen

Article

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

EGLN2 (EGLN2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-CUL1 K720A

Plasmid

#19939

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 K720A (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

CUL1 with a NEDD8 modification mutant

Available Since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
MSCV FLIPi P2G_Thy1.1 p53

Plasmid

#19748

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 (Trp53 Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pQCXIP Flag-hRpt6

Plasmid

#19793

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

hRpt6 (PSMC5 Human)

Use

Retroviral

Tags

Flag

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pCMV5 DPC4 (1-514)

Plasmid

#14040

Depositor

Joan Massague

Article

Lagna et al Nature. 1996 Oct 31. 383(6603):832-6.

Insert

DPC4 (1-514) (SMAD4 Human)

Expression

Mammalian

Mutation

R515 was mutated into a stop codon. AGA to TGA. D…

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

Plasmid

#172982

Purpose

control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

control sgRNA

Use

CRISPR and Lentiviral

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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WT GLUT1-GFP

Plasmid

#200093

Purpose

WT human GLUT1 with a C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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PWPXLD-EGFR-WT

Plasmid

#133749

Purpose

Wild-type EGFR tagged with HA cloned into lentiviral PWPXLD plasimd

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

Wild type EGFR-HA (EGFR Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro dr_cPLA2-mKate2

Plasmid

#163844

Purpose

Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMito-mCherry-FRB_IRES_FKBP(I)-GBPen

Plasmid

#128267

Purpose

Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 1x FKBP-GBPenhancer (GFP Nanobody with 1 FKBP domain).

Depositor

Stephen Royle

Article

Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

Inserts

MitoTrap
FKBP(I)-GBPen

Expression

Mammalian

Promoter

CMV and IRES

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP-proα2(I)

Plasmid

#119826

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING HAQ

Plasmid

#102600

Purpose

Express STING HAQ for IFN-beta Luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R71H-G230A-R293Q (HAQ)

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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