We narrowed to 15,882 results for: LEA
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Plasmid#163710PurposeYeast low copy plasmid with dCas9 and sgRNA expression cassetteDepositorInsertdCas9
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GR-ICUE2
Plasmid#173017PurposeImproved green-red FRET based cAMP indicator.DepositorInsertGR-ICUE2
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETZZtevPP7CP(C68A,C73A)
Plasmid#27548DepositorInsertPseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
TagsTEV protease cleavage site, six histidine tag, an…ExpressionBacterialMutationchanged two cysteine to alanine (C68A,C73A) to in…Available SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
mini-donor - SOX17-EGFP
Plasmid#206048Purposethis mini-donor knockin vector can be used to knock EGFP into SOX17 locusDepositorInsertnuclear EGFP
UseKnockin donor vectorAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-StagRFP
Plasmid#173014PurposeRationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.DepositorInsertStagRFP
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianPromoterCMVAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAPV-DsRed2-ER-5
Plasmid#170570PurposeExpresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.DepositorInsertAPV-DsRed2-ER-5
ExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a - TR
Plasmid#89621Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG inductionDepositorInsertThioredoxin
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyCatcher
Plasmid#72326PurposeBacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.DepositorInsertAviTag-SpyCatcher
TagsN-term His6 tag and TEV cleavage siteExpressionBacterialAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pelBHisTEV
Plasmid#157741Purposeempty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sitesDepositorTypeEmpty backboneTagsPelB signal sequence + His10-tag + TEV protease c…ExpressionBacterialPromoterT7lacAvailable SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Parp14-c012
Plasmid#173099PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-CFP
Plasmid#74151PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker CeruleanDepositorInsertTol2-kanaR-cryaa-CFP
Available SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Parp14-c010
Plasmid#173100PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVTU_260_BirA
Plasmid#63682PurposeEncodes E. coli biotin ligase gene (BirA) for expression in yeast cellsDepositorInsertBirA
Tags6X Histidine and TEV cleavage siteExpressionYeastPromoterADH1/yeastAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb
Plasmid#175534PurposeExpression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag7-myc_PDGFRtmb
ExpressionMammalianAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBad-UGAcS
Plasmid#172137Purposebacteria expression plasmid for a UDP-GlcNAc sensorDepositorInsertUGAcS
ExpressionBacterialMutationH605Q- please see depositor commentsPromoteraraBADAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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5UAS zfSynaptophysin:GFP
Plasmid#74316PurposeA fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeatsDepositorInsertsynaptophysin
TagsEGFPMutationQ12H and deletion of F13 (please see depositor co…Promoter5UASAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
FnR8-10
Plasmid#182569PurposeHuman fibronectin fragment, type-III repeats 8-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
TagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379AAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
HECTD1
Plasmid#32860PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHECTD1 (HECTD1 Human)
TagsHis (with TEV cleavage)ExpressionBacterialMutationamino acids 1879-1966 onlyAvailable SinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-H2B_Halo_T2A_EGFP
Plasmid#135444PurposeMamalian cell expression of nuclear HaloTag7 and cytosolic EGFPDepositorInsertH2B-HaloTag7 and EGFP
TagsH2BExpressionMammalianPromoterCMV/TetOAvailable SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only