We narrowed to 14,473 results for: cas9 genes

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px459V2.0_Conc3

Plasmid

#134458

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMJ826

Plasmid

#39316

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Histidine 840 to Alanine (H840A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pTJV1Sc3-rpoB

Plasmid

#166983

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Insert

RecT (recT E. coli)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pVvanCC

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSCL.140

Plasmid

#184995

Purpose

Express Cas9 in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

SpCas9

Tags

SV40NLS

Expression

Mammalian

Promoter

TetOn-3g

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSDMA65

Plasmid

#128354

Purpose

Codon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.

Depositor

James Fraser

Article

Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

Type

Empty backbone

Use

CRISPR

Promoter

ACT1

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1.mir451_KO

Plasmid

#164689

Purpose

For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

miR-451 CRISPR KO gRNA (MIR451A )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCZGY2729

Plasmid

#135096

Purpose

Site specific insertion using CRISPR/Cas9 editing of C. elegans ChrIV

Depositor

Yishi Jin

Article

Andrusiak et al Neuron. 2019 Jul 24. pii: S0896-6273(19)

Insert

Hygromycin resistance

Use

CRISPR

Expression

Worm

Promoter

rps-0

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Drosha_4

Plasmid

#79863

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Drosha_1

Plasmid

#79860

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Drosha_2

Plasmid

#79861

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Drosha_3

Plasmid

#79862

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
hIRF-1 gRNA #351 (Sa)

Plasmid

#105283

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #351

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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hIRF-1 gRNA #409 (Sa)

Plasmid

#98134

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #409

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pDGE168

Plasmid

#79458

Purpose

recipient plasmid [multiplexing]; pDD45:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGEF-U

Plasmid

#251731

Purpose

Geminivirus derived-CRISPR/Cas9 plasmid with GFP for cloning of up to four gRNAs

Depositor

Humberto Prieto

Article

et al Agronomy 2026, 16, 216. https://doi.org/

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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pVH-U

Plasmid

#252743

Purpose

Geminivirus derived-CRISPR/Cas9 plasmid cloning of up to four gRNAs. Encodes GFP and hygromycin resistance.

Depositor

Humberto Prieto

Article

et al Agronomy 2026, 16, 216. https://doi.org/

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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pVK-U

Plasmid

#252744

Purpose

Geminivirus derived-CRISPR/Cas9 plasmid cloning of up to four gRNAs. Encodes GFP and kanamycin resistance.

Depositor

Humberto Prieto

Article

et al Agronomy 2026, 16, 216. https://doi.org/

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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pPRISM-QF2-he1.1:YFP

Plasmid

#184819

Purpose

Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QF2-pA-he1.1:YFP

Use

CRISPR; Backbone for cloning of homology arms (fo…

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target1 (Mpphot)

Plasmid

#186728

Purpose

Binary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Expression

Bacterial

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target2 (Mpphot)

Plasmid

#186729

Purpose

Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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