We narrowed to 15,772 results for: LEA

1. pCMVMagFRET-1-NLS

   Plasmid

   #50748

   Purpose

   Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

   Depositor

   Maarten Merkx

   Article

   Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

   Insert

   gene encoding for MagFRET-1 with NLS
   

   Expression

   Mammalian

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET26b_Nb.AT110

   Plasmid

   #135480

   Purpose

   E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

   Depositor

   Andrew Kruse

   Article

   Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

   Insert

   Nanobody AT110
   

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_449

   Plasmid

   #179093

   Purpose

   nuclease Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   SpG
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCC_083 Cas12i1

   Plasmid

   #171670

   Purpose

   For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   Cas12i1
   

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAD6

   Plasmid

   #134349

   Purpose

   E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

   Depositor

   Seth Darst

   Article

   Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

   Insert

   mfd (mfd E. coli)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFBNb-cALFA

   Plasmid

   #159990

   Purpose

   For the protein expression with ALFA tag at C-terminus in insect cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Insect

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. KEAP1 (KEAP1A-c029)

   Plasmid

   #110247

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KEAP1 (KEAP1 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7 - lacO

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA-FRT-FAK30

   Plasmid

   #59121

   Purpose

   FAK fret sensor with 30 nm helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. DOT1L

   Plasmid

   #36196

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   DOT1L (GeneScript Synthesized) (DOT1L Human)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   contains amino acid residues 1-420

   Available Since

   Aug. 8, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

10. NES-iRFP-P4Mx1

    Plasmid

    #108144

    Purpose

    PI4P biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    X.leavis map2k1.L(32-44):iRFP:L. penomophila SidM(546-647)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. JMJD1CA-c089

    Plasmid

    #79644

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JMJD1C (JMJD1C Human)
    

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMH220

    Plasmid

    #122836

    Purpose

    dCas9 with GreenGate B-C flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    

    Use

    Golden gate compatible cloning vector

    Mutation

    Contains D10A and H840A mutations

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPR_full-site

    Plasmid

    #186277

    Purpose

    Reporter plasmid for full-site selection assay

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
    

    Expression

    Bacterial

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2-FAK30

    Plasmid

    #59131

    Purpose

    Cloning vector for FAK fret sensor with 30 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA-FRT-FAK10

    Plasmid

    #59119

    Purpose

    FAK fret sensor with 10 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. MXS_P2A

    Plasmid

    #62433

    Purpose

    MXS_chaining vector with P2A

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Self-cleaving peptide
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. csr-1_son-1_mTagBFP2

    Plasmid

    #191746

    Purpose

    Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    NCU04288 (NCU04288 Neurospora crassa)
    

    Tags

    NCU04502 promoter and mTagBFP2

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. CBX4

    Plasmid

    #25237

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX4_02 (CBX4 Human)
    

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pQE30-TEV-pro-conA

    Plasmid

    #159527

    Purpose

    Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.

    Depositor

    Joshua Mylne

    Article

    Nonis et al bioRxiv. 2020.

    Insert

    Canavalia ensiformis pro-concanavalin A
    

    Tags

    Six-Histidine tag and tobacco etch virus (TEV) pr…

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV10-3xFLAG-prs-MCP1

    Plasmid

    #184341

    Purpose

    Express yeast MCP1 in Expi293F cells

    Depositor

    Karin Reinisch

    Article

    Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

    Insert

    Yeast MCP1 (MCP1 Budding Yeast)
    

    Tags

    3xFLAG-PreScission cleavage site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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