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pRSFDuetP-HsEDC3_192-228-GST-HsRCK-E236Q_E

Plasmid

#146212

Purpose

Bacterial Expression of HsEDC3_192-228-HsRck-E236Q

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-228-HsRck-E236Q (EDC3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-228-GST-HsRCK-K135A_E

Plasmid

#146213

Purpose

Bacterial Expression of HsEDC3_192-228-HsRck-K135A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-228-HsRck-K135A (EDC3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508-GST-HsRCK-E236Q_E

Plasmid

#146215

Purpose

Bacterial Expression of HsEDC3_192-508-HsRck-E236Q

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-508-HsRck-E236Q (EDC3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508-GST-HsRCK-K135A_E

Plasmid

#146216

Purpose

Bacterial Expression of HsEDC3_192-508-HsRck-K135A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-508-HsRck-K135A (EDC3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-Q281AH284A_E

Plasmid

#146204

Purpose

Insect Expression of DmMe31B-Q281AH284A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmMe31B-Q281AH284A (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G and two non silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-T288AK292A_E

Plasmid

#146205

Purpose

Insect Expression of DmMe31B-T288AK292A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmMe31B-T288AK292A (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G and two non silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GA46

Plasmid

#196614

Purpose

Plasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

ATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pShuttle.CC9

Plasmid

#192931

Purpose

Gateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expression

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Type

Empty backbone

Use

Gateway-compatible cloning vector

Promoter

CMV

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

Plasmid

#186703

Purpose

Mammalian expression of Tiam1(DH-PH) fused to miRFP703-eDHFR(69K6)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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eDrMet

Plasmid

#183999

Purpose

Optically controlled eDrcMet expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrMet (MET Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_Chromo-W35A_TAF3_Phd

Plasmid

#179401

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo(W35A) - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo(W35A) - TAF3_Phd

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Chromodomain W35A

Promoter

CAGGS

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Flag-Mcm2 targeting sgRNA

Plasmid

#186937

Purpose

Genomic targeting of Flag tag at Mcm2 N-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Flag-Mcm2 targeting sgRNA (Mcm2 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Mcm2-2A mutation sgRNA

Plasmid

#186936

Purpose

sgRNA used for genetic mutation at Y81 and Y90 of Mcm2

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Mcm2-2A mutation sgRNA (Mcm2 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-742pUC19-tNGFR-P2A-Caspase8

Plasmid

#186099

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CASPASE8

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTOPO-col10a1-KI-donor

Plasmid

#184874

Purpose

Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair

Depositor

Christoph Winkler

Article

Hui et al Development. 2022 May 20. pii: 275483. d

Inserts

Collagen10a1 5' homology arm
Collagen10a1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
eGFP

Use

CRISPR and Cre/Lox

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.EPC1

Plasmid

#184653

Purpose

Retroviral expression of mouse Epc1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc1 (Epc1 Mouse)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.EPC2

Plasmid

#184654

Purpose

Retroviral expression of mouse Epc2

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc2 (Epc2 Mouse)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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Dendra-Rab11a

Plasmid

#184038

Purpose

Expresses red to green photoconvertible Rab11a

Depositor

Heidi Hehnly

Article

Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

Insert

Rab11 (RAB11A Human)

Tags

Dendra

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pPOLY_new

Plasmid

#179276

Purpose

Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

Depositor

Bernd Nidetzky

Article

Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

Inserts

cellodextrin phosphorylase
cellobiose phosphorylase
sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)

Use

Synthetic Biology

Tags

6xHIS and Strep-Tag II

Expression

Bacterial

Promoter

T7 lacO

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBICI_high ori

Plasmid

#179273

Purpose

Expresses CuCBP and BaSP in E. coli BL21(DE3)

Depositor

Bernd Nidetzky

Article

Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Promoter

T7 lacO

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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