We narrowed to 60,318 results for: cloning
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Plasmid#79022PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only
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pAS2-his-24::tagRFP
Plasmid#124330PurposeExpressing Nuclear Localized tagRFPDepositorInserttagRFP (his-24 Nematode)
ExpressionWormAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10 His6-MBP TEV Ade S. cerevisiae expression vector (12ADE-C)
Plasmid#48299DepositorTypeEmpty backboneUseNaTagsHis6 and MBPAvailable SinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Nup133_mEGFP(-9a)
Plasmid#163419PurposeMammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)DepositorAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shRen-Blast
Plasmid#135684PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selectionDepositorInsertshRenilla
UseLentiviralAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-tdTomato-miRE_shRen-Blast
Plasmid#135686PurposeConstitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selectionDepositorInsertshRenilla
UseLentiviralAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_50_100_CA_repeat
Plasmid#65867PurposeCarries CA repeats of 50-100 nucleotides in length, an RBPMS targetDepositorInsertCA repeats 50-100 nucleotides in lngth
PromoterT7Available SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
UC20m
Plasmid#121040PurposeMoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).DepositorInsertgN2 Cas9 gRNA UC part
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC49
Plasmid#104823PurposeCRISPR/Cas9 2xplex gRNA targeting Glyma17g11235 (Dcl4a). Also expresses Cas9 from AtUBQ10 promoter.DepositorInsertGlyma17g11235
UseCRISPRExpressionPlantAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PARP14A
Plasmid#42487PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPARP14A
TagsHis6-TEVExpressionBacterialMutationSee CommentsAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pREP3xN_ccdB
Plasmid#79021PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEos-A69V-C1
Plasmid#54647PurposeLocalization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581DepositorTypeEmpty backboneTagsmEos A69VExpressionMammalianAvailable SinceJune 11, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
C71m
Plasmid#120983PurposeMoClo golden gate assembly CD part for CinR-CIDDHYRTC n-2 (Adam Meyer's improved CinR activator from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).DepositorInsertCinR Adam Meyer
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTMR6A
Plasmid#42483PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMTMR6A
TagsHis6ExpressionBacterialMutationSee CommentsAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-Epo
Plasmid#114290PurposeTET-inducible expression of ErythropoietinDepositorInsertErythropoietin (Epo Rat)
UseLentiviralAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
INPP5EA
Plasmid#42481PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertINPP5EA
TagsHis6ExpressionBacterialMutationSee CommentsAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAS1-his-24::Cerulean
Plasmid#124323PurposeExpressing Nuclear Localized CeruleanDepositorInsertCerulean (his-24 Nematode)
ExpressionWormAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
PARP14A
Plasmid#42488PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPARP14A
TagsHis6-TEVExpressionBacterialMutationSee CommentsAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
PARP14A
Plasmid#42489PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPARP14A
TagsHis6-TEVExpressionBacterialMutationSee CommentsAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTUB:FLuc
Plasmid#114578PurposeConstitutive expression of firefly luciferase in T. gondii using the alpha tubulin promoter; floxed pyrimethamine selectable marker; UPRT locus flanking sequence to direct integrationDepositorInsertFirefly Luciferase
UseCre/Lox and LuciferasePromoteralpha tubulin T. gondiiAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only