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pcDNA-ApplePy1High

Plasmid

#239065

Purpose

Mammalian expression of high affinity red fluorescent pyruvate biosensor ApplePy1High

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

ApplePy1High

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B1_M13ps

Plasmid

#231422

Purpose

M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B1

Use

Synthetic Biology

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-2_M13ps

Plasmid

#231412

Purpose

M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-2

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-S1S2 [rev-backbone]

Plasmid

#229199

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK74

Plasmid

#226657

Purpose

Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, KanR, contains HIP1 site

Depositor

David Nielsen

Article

Kamoku et al Synthetic Biology and Engineering 2024,

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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scFv-SmBiT

Plasmid

#192541

Purpose

Expresses sender cell components of LOTIIS

Depositor

Jennifer Prescher

Article

Ng et al Bioconjug Chem. 2022 Oct 19;33(10):1876-

Insert

scFv - SmBiT86

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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2x GCN4-LgBiT

Plasmid

#192542

Purpose

Expresses receiver cell components of LOTIIS

Depositor

Jennifer Prescher

Article

Ng et al Bioconjug Chem. 2022 Oct 19;33(10):1876-

Insert

2x GCN4-LgBiT

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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neg.ctrl_pSTC0-zeo

Plasmid

#202350

Purpose

Control neg. ctrl (negative control S70A) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

neg. ctrl

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJT87

Plasmid

#204489

Purpose

CAG promoted WBeta recombinase

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Insert

WBeta recombinase

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS100

Plasmid

#209354

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-B'-l2-B'

Plasmid

#209119

Purpose

Bacterial expression of CC-GEMS ligand SUMO-B'-l2-B'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-B'-l2-B'

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pJT88

Plasmid

#204490

Purpose

CAG promoted phiC31 recombinase

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Insert

phiC31 recombinase

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l3-A'

Plasmid

#209118

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l3-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l3-A'

Expression

Bacterial

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l2-G'

Plasmid

#209122

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l2-G'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-G'

Expression

Bacterial

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l2-B'

Plasmid

#209121

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l2-B'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-B'

Expression

Bacterial

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l1-A'

Plasmid

#209116

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l1-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l1-A'

Expression

Bacterial

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-G'-l2-G'

Plasmid

#209120

Purpose

Bacterial expression of CC-GEMS ligand SUMO-G'-l2-G'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-G'-l2-G'

Expression

Bacterial

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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