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JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

Plasmid

#156405

Purpose

Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4A-WT (KRAS Human)

Use

Gateway cloning

Tags

mScarlet-I

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pVD1 tRNA-gRNA position E3 (GB2240)

Plasmid

#160562

Purpose

tRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

tRNA-gRNA position E3 (Multiplexing Edit)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 rtTA BirA eIF4G

Plasmid

#158789

Purpose

pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1

Tags

3xFlag and BirA*

Expression

Mammalian

Promoter

miniCMV

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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HisGB1-CITED4(130-184) + CBP TAZ1(340-439)

Plasmid

#254288

Purpose

Bacterial coexpression of human CITED4 CTAD with CBP TAZ1

Depositor

Rebecca Berlow

Article

Do et al bioRxiv [Preprint]. 2026 Jan 21:2026.01.

Inserts

CITED4 (130-184) (CITED4 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Tags

6X His-tagged GB1

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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HisGB1-CITED2(216-270) + CBP TAZ1(340-439)

Plasmid

#254287

Purpose

Bacterial coexpression of human CITED2 CTAD with CBP TAZ1

Depositor

Rebecca Berlow

Article

Do et al bioRxiv [Preprint]. 2026 Jan 21:2026.01.

Inserts

CITED2 (216-270) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Tags

6X His-tagged GB1

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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HisGB1-CITED1(146-193) + CBP TAZ1(340-439)

Plasmid

#254286

Purpose

Bacterial coexpression of human CITED1 CTAD with CBP TAZ1

Depositor

Rebecca Berlow

Article

Do et al bioRxiv [Preprint]. 2026 Jan 21:2026.01.

Inserts

CITED1 (146-193) (CITED1 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Tags

6X His-tagged GB1

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_AlphaSKE_ALFA_231232

Plasmid

#250444

Purpose

Expresses tagged alpha skeletal muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T231/A232.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Alpha Skeletal Muscle Actin (ACTA1 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_AlphaCARD_ALFA_231232

Plasmid

#250443

Purpose

Expresses tagged alpha cardiac muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T231/A232.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Alpha Cardiac Muscle Actin (ACTC1 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_GammaSMA_ALFA_230231

Plasmid

#250442

Purpose

Expresses tagged gamma smooth muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T230/A231.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Gamma Smooth Muscle Actin (ACTG2 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_GammaActin_ALFA_229230

Plasmid

#250440

Purpose

Expresses tagged gamma cytoplasmic actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T229/A230.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Gamma Cytoplasmic Actin (ACTG1 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_BetaActin_ALFA_229230

Plasmid

#250439

Purpose

Expresses tagged beta cytoplasmic actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T229/A230.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Beta Cytoplasmic Actin (ACTB Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-sGPR124-VEGFR2 TMD-FKBP

Plasmid

#246697

Purpose

Expression vector for human GPR124 ECD fused to human VEGFR2 TMD and human FKBP1A F36V, N-terminal 3xFLAG tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and hVEGFR2 TMD-hFKBP1A F36V

Expression

Mammalian

Mutation

ECD

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-ANO9/TMEM16J [N443/17R]

Plasmid

#222164

Purpose

Mammalian Expression Plasmid of anti-ANO9/TMEM16J (Human). Derived from hybridoma N443/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-ANO9/TMEM16J (Homo sapiens) recombinant mouse monoclonal antibody. (ANO9 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-M1-BRG1-3xFLAG-BAF57

Plasmid

#109189

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged BRG1 and untagged BAF57 under the control of a p10 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

Inserts

BRG1 (SMARCA4 Human)
BAF57 (SMARCE1 Human)

Tags

3xFLAG

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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ABL1-C-HiBiT

Plasmid

#234568

Purpose

Bacterially expressed ABL1-C-HiBiT for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

Abelson Kinase Domain (ABL1 Human)

Tags

HiBiT Tag-thrombin site-10xHis and T7 gene 10 lea…

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

T7

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLJM1-NRBP1-Cterm-C-FLAG

Plasmid

#222679

Purpose

overexpressing C-terminus truncation of NRBP1 with C-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

NRBP1 (NRBP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

NRBP1 C-terminus truncation

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pdest myr-AKT2 puro

Plasmid

#223053

Purpose

myr-AKT2 gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

myr-AKT2 (Akt2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-S27A Neo

Plasmid

#223059

Purpose

METTL1-S27A gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27A (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S27A

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-S27D Neo

Plasmid

#223060

Purpose

METTL1-S27D gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27D (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S27D

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdest myr-AKT2 Neo

Plasmid

#223063

Purpose

myr-AKT2 gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

myr-AKT2 (Akt2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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