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pTIM

Plasmid

#81198

Purpose

pTIM in vitro transcription backbone plasmid with two multiple cloning sites and two Rho-independent transcriptional terminators.

Depositor

Andrés Vázquez-Torres

Article

Tapscott et al Sci Rep. 2018 Jun 21;8(1):9465. doi: 10.

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

Plasmid

#72611

Purpose

For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

changed serine33, threonine34 and serine37 to ala…

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
MT79

Plasmid

#63112

Purpose

The artificial microRNA is designed for down-regulating expression of ACD6 mRNA.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

amiR-ACD6

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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Mouse Nanog locus 3' probe

Plasmid

#60037

Purpose

Cloned genomic fragment of murine Nanog locus used as probe for Southern Blot analysis

Depositor

Jacob Hanna

Article

Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

Insert

Nanog (Nanog Mouse)

Use

Unspecified; Cloning

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pTYB-pLAC-I68S/I73S

Plasmid

#48728

Purpose

Bacterial expression of Lacritin Proteins with Mutation at protein 68 and 73 from Isoleucine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Isoleucine 68 and 73 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB-pLAC-F112S

Plasmid

#48727

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Phenylalanine 12 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB-pLAC-L108S

Plasmid

#48725

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 108 from Leucine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Leucine 108 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-I98S

Plasmid

#48723

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 98 from Isoleucine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Isoleucine 98 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-I73S

Plasmid

#48722

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 73 from Isoleucine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Isoleucine 73 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-N71

Plasmid

#48720

Purpose

Bacterial expression of Lacritin protein with 70 AA removed from the N-terminus

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Deleted first 71 amino acids of mature lacritin w…

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-N45

Plasmid

#48718

Purpose

Bacterial expression of Lacritin protein with 45 AA removed from N terminus

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Deleted first 45 amino acids of mature lacritin w…

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-V69S

Plasmid

#48721

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 69 from Valine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Valine 69 to Serine

Promoter

T7

Available Since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-dTomato-ORP9-PH

Plasmid

#214268

Purpose

Mammalian expression of red fluorescent PI4P reporter dTomato-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

dTomato-ORP9(1-111) (Osbpl9 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD1

Plasmid

#195839

Purpose

Expresses human FZD1 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD1 (FZD1 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-143

Plasmid

#118559

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBBR5pemIK

Plasmid

#126554

Purpose

Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection

Depositor

Lindsey Burbank, Drake Stenger

Article

Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

Type

Empty backbone

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-140

Plasmid

#118524

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-150

Plasmid

#118532

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE161

Plasmid

#118509

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE146

Plasmid

#118498

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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