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pAIP4_3RZY
Plasmid#234156PurposeHeterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coliDepositorInsert3RZY (FABP4 Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAP21
Plasmid#171207Purposebacterial expression for HIS purification of the MEG-3 C-terminus with mutations in the HMG-like motifDepositorInsertmeg-3 (meg-3 Nematode)
UseTags8xHISExpressionBacterialMutationaa545-862, F705A,Y708A,Y713A, N725APromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
GGDest2-Amp
Plasmid#157650PurposeGolden Gate assembly of a pET28-compatible fusion-protein ORF insertDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoternoneAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSP850
Plasmid#98797PurposeExpression VectorDepositorInsertpET28 MBP-HPLVGHM-TEV L1F 111215
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP851
Plasmid#98798PurposeExpression VectorDepositorInsertpET28 MBP-HPLVGHM-TEV L2F 111215
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP852
Plasmid#98799PurposeExpression VectorDepositorInsertpET28 MBP-HPLVGHM-TEV L5B 111215
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP968
Plasmid#98800PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQM-TEV H28L T30A
UseTagsExpressionBacterialMutationH28L, T30APromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
UBH12 (3DB4)
Plasmid#25171PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertubh12.414.617:B9 (UHRF1 Human)
UseTagsHisExpressionBacterialMutationcontains residues 126-285PromoterAvailable sinceApril 4, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
TJP3
Plasmid#25310PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTJP3:G508-L808:cH6 (TJP3 Human)
UseTagsHisExpressionBacterialMutationContains amino acids G508-L808PromoterAvailable sinceApril 4, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJK652
Plasmid#72470PurposeProduces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)DepositorInsertacetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationMutated histidine-233 to glycinePromoterT7Available sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK288
Plasmid#71552PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with Tyr260>Ala mutant (AaAlr-Y260A)DepositorInsertalanine racemase
UseTagsExpressionBacterialMutationchanges tyrosine-260 to alaninePromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK301
Plasmid#71555PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with C-terminal His tag (AaAlrH6)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK559
Plasmid#71603PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, tyrosine-…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK562
Plasmid#71604PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, tyrosine-…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK604
Plasmid#71610PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe mutant (AaPurE1fww-H59N,Y154F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, histidine…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK664
Plasmid#71613PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-H59N,Y154F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparagine, tyrosine-154 …PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK666
Plasmid#71615PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparagine, tryptophan-97…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK563
Plasmid#71689PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant (AaPurE1fwf-Y154I)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, tyrosine-…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK508
Plasmid#71598PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp165>Phe mutant (AaPurE1fwf-H59N)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, histidine…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP-MAPK2 (3RP9)
Plasmid#32862PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAPK2
UseTagsHis tag with TEV cleavageExpressionBacterialMutationamino acids 123-548 only, P421LPromoterAvailable sinceJan. 10, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
TDRD7 (3RCO)
Plasmid#32051PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTDRD7 (TDRD7 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
SGF29 (3MEU)
Plasmid#25317PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSGF29_6 (SGF29 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 115-293PromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
SGF29 (3MEW)
Plasmid#25320PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSGF29_23 (SGF29 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 129-291PromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
SGF29 (3LX7)
Plasmid#25314PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSGF29_3 (SGF29 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 138-291PromoterAvailable sinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
C6orf197
Plasmid#25258PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertC6orf197:M178-R505 (TBC1D22B Human)
UseTagsHisExpressionBacterialMutationContains amino acids M178-R505PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PLXND1 (3H6N)
Plasmid#25187PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPLXND1:A1553-D1656 (PLXND1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids A1553-D1656PromoterAvailable sinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pET_mNG2(1-10)_32aalinker_mNG2(11)
Plasmid#82611PurposeBacterial expression vector for split mNeonGreen2 screeningDepositorInsertmNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
UseTagsExpressionBacterialMutationsplit before mutationsPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSAB35
Plasmid#175787PurposeExpresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tagDepositorInsertSidK (1-278)
UseTags3xFLAG and 6xHis TEVExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET_RTX-6xHis
Plasmid#145029PurposepET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tagDepositorArticleInsertRTX
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p7XNH3
Plasmid#47064PurposeFX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionBacterialMutationPromoterT7Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
USP21 (3I3T)
Plasmid#25147PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertusp21.209.563 (USP21 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSANG10-3F
Plasmid#39264DepositorTypeEmpty backboneUseBacterial expression, bacterial expression of re…Tags6xHis, pelB leader, and tri-FLAGExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3990 pET ubcH8
Plasmid#8650DepositorInsertUbcH8 (UBE2L6 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
4RCI
Plasmid#64653PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertYTHDF1:JMC02A-E04:C231829 (YTHDF1 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceApril 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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5W9Q (MBD1)
Plasmid#101265PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMBD1 (MBD1 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
p7XC3H
Plasmid#47065PurposeFX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionBacterialMutationPromoterT7Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
6C1A (MBD2)
Plasmid#119958PurposeBacterial expression for structure determinationDepositorInsertMBD2 (MBD2 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET_scFv_QLuc
Plasmid#183261PurposeExpress scFv_QLuc in bacteria cellsDepositorInsertscFv_QLuc
UseTagsHistagExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
UHRF1
Plasmid#28163PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUHRF1 (UHRF1 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationtandem tudor domainPromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
AD-003
Plasmid#25502PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAD-003 (NTMT1 Human)
UseTags6x His and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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PaBphP 1-497
Plasmid#36920DepositorInsertPaBphP-PCD
UseTagsHisExpressionBacterialMutationContains the photosensory core domain coding regi…PromoterT7Available sinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
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MSP577
Plasmid#98801PurposeExpression VectorDepositorInsertpET28 MBP-HNLYFQS-TEV I138T, N171D, N176T
UseTagsExpressionBacterialMutationI138T, N171D, N176TPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK295
Plasmid#71561PurposeProduction of Acetobacter aceti 1023 thioredoxin (AaTrx)DepositorInsertthioredoxin
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only
