We narrowed to 31,887 results for: Eng

1. TS-PE2-N

   Plasmid

   #176649

   Purpose

   Expresses N-terminus of PE2 in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

   Inserts

   N-terminus of PE2
   Artificial SD
   

   Use

   AAV

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV and None

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Linked Free NES

   Plasmid

   #182485

   Purpose

   Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Insert

   FPPS-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Linked Free PcPTS

   Plasmid

   #182486

   Purpose

   Yeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Insert

   FPPS-PTS
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Coexpressed Free GFP-NES

   Plasmid

   #182491

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   GFP-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10 and GAL7

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV_Npu-saBE3.9max_KKH C-terminal

   Plasmid

   #137181

   Purpose

   Expresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-saBE3.9max_KKH_C-terminal
   

   Expression

   Mammalian

   Mutation

   E782K;N968K;R1015H conferring recognition of NNNR…

   Promoter

   CMV

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZ_P-GAP-LacI-Mit1AD

   Plasmid

   #126722

   Purpose

   LacI-Mit1AD on pGAPZ_ A expression vector

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   hybrid lacI-Mit1 activation domain coding sequence
   

   Expression

   Yeast

   Promoter

   GAP

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWT-IFl_WT-IFr

   Plasmid

   #192207

   Purpose

   Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Wildtype I-F Leader and wildtype I-F Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pScr-IFl_WT-IFr

   Plasmid

   #192208

   Purpose

   Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Scrambled I-F Leader and wildtype I-F Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCon-IFl_Con-IFr

   Plasmid

   #192209

   Purpose

   Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus I-F Leader and Consensus I-F Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCon-IFl_Con-IEr

    Plasmid

    #192210

    Purpose

    Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Consensus I-F Leader and Consensus I-E Locus DNA
    

    Expression

    Bacterial

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCon-IFl_Con-ICr

    Plasmid

    #192211

    Purpose

    Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Consensus I-F Leader and Consensus I-C Locus DNA
    

    Expression

    Bacterial

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCon-IFl_Con-IIAr

    Plasmid

    #192212

    Purpose

    Consensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Consensus I-F Leader and Consensus II-A Locus DNA
    

    Expression

    Bacterial

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCon-IEl_Con-IEr

    Plasmid

    #192213

    Purpose

    Consensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Consensus I-E Leader and Consensus I-E Locus DNA
    

    Expression

    Bacterial

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCon-ICl_Con-ICr

    Plasmid

    #192214

    Purpose

    Consensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Consensus I-C Leader and Consensus I-C Locus DNA
    

    Expression

    Bacterial

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCon-IIAl_Con-IIAr

    Plasmid

    #192215

    Purpose

    Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Consensus II-A Leader and Consensus II-A Locus DNA
    

    Expression

    Bacterial

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX1-IFl_Con-IFr

    Plasmid

    #192216

    Purpose

    Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    I-F leader and I-F repeat construct not studied further in this paper (X1)
    

    Expression

    Bacterial

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX2-IFl_Con-IFr

    Plasmid

    #192217

    Purpose

    Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    I-F leader and I-F repeat construct not studied further in this paper (X2)
    

    Expression

    Bacterial

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCHC030 (∆CBBp ∆MBH ∆SH)

    Plasmid

    #226205

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for CBBp, MBH, and SH operons, and intervening sequencessequences.
    

    Use

    Synthetic Biology

    Mutation

    ∆cbbR ∆cbbLSXYEFPTZGKA ∆hoxKGZMLOQRTV ∆hypA1B1F1C…

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCHC036 (∆pHG1)

    Plasmid

    #226206

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for the pHG1 megaplasmid replication region
    

    Use

    Synthetic Biology

    Mutation

    ∆helD ∆repA ∆repB ∆parAB

    Available Since

    Nov. 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pACB120

    Plasmid

    #222213

    Purpose

    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag

    Depositor

    Gregg Beckham

    Article

    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

    Insert

    fghA(K10N, S11R, W15C, G76V, G258D)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Contains five amino acid mutations: K10N, S11R, W…

    Promoter

    T7

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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