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TRPM7 gRNA (BRDN0001146152)

Plasmid

#76111

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM7 (TRPM7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K8 gRNA (BRDN0001146835)

Plasmid

#75923

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K8 (MAP3K8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HSPB8 gRNA (BRDN0001145453)

Plasmid

#75640

Purpose

3rd generation lentiviral gRNA plasmid targeting human HSPB8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HSPB8 (HSPB8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CCL2 gRNA (BRDN0001147233)

Plasmid

#77865

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCL2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CCL2 gRNA (CCL2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75238

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75239

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-mNanog-mut-pgk-hph

Plasmid

#74914

Purpose

Mammalian expression of mNanog (Y42E, K43T)

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Mutation

K43T of Nanog protein and Y42E of Nanog homeodoma…

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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hPlekhm1-C/pIRES puro Glue (H987-end)

Plasmid

#73458

Purpose

Express human C-terminal part (H987-end) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-C/pIRES puro Glue (S778-Q986)

Plasmid

#73457

Purpose

Express human C-terminal part (S778-Q986) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB2a Cterminal tail

Plasmid

#60396

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP TEM4 DEL NTERM

Plasmid

#58894

Purpose

Expresses GFP tagged TEM4 with N terminus deleted

Depositor

Channing Der

Article

Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

Insert

TEM4 (ARHGEF17 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deleted N Terminus up to aa 1002

Promoter

CMV

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
p6615 MSCV-IP N-HAonly NIPA1

Plasmid

#35013

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

NIPA1 (RBCK1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/Flag-hINO80C (1224-1556)

Plasmid

#29445

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 1224-1556 (a.a.)

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
pM-ErbB4CTF-PY1 mutant

Plasmid

#17798

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 676-1292, …

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pM-ErbB4CTF-PY3 mutant

Plasmid

#17799

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 676-1292, …

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L16A

Plasmid

#13476

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L16A (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 16 to Alanine

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L17A

Plasmid

#13477

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L17A (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 17 to Alanine

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pk335-CARGO-12mer-14kb-USF-1-12

Plasmid

#227472

Purpose

12-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 14kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET19pps-EndoIV(Thermus Thermophilus)

Plasmid

#236048

Purpose

pET19pps-APE1(Thermus Thermophilus)

Depositor

Luis Gabriel Brieba

Article

Cordoba-Andrade et al PeerJ. 2025 Aug 13;13:e19758. doi: 10.77

Insert

Endo IV from Thermus thermophilus

Tags

His tag and Histidine tag

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

Aug. 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTRIP-SFFV-EGFP-FLAG-UL36USP (C65)

Plasmid

#235549

Purpose

To express GFP-FLAG-UL36USP (C65) - region 1-520 of UL36

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

UL36USP (C65)

Use

Lentiviral

Tags

EGFP-FLAG

Mutation

N-terminal part of UL36 starting at the first ATG…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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