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pCDH-3xFLAG-GFP-puroR

Plasmid

#167463

Purpose

3rd-gen lentiviral expression of FLAG-GFP in mammalian cells

Depositor

Steven Artandi

Article

Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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JD150

Plasmid

#229485

Purpose

Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E2A, VA RNA I, E4orf6, L4-22k/33k

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-DIO-mNeonGreen-WPRE

Plasmid

#223669

Purpose

Cre-dependently expresses mNeonGreen in astorocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mNeonGreen

Use

AAV and Cre/Lox

Promoter

GfaABC1D

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pTSAR3.5t

Plasmid

#210257

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

eBFP2
superfolder GFP

Tags

ssra

Expression

Bacterial

Promoter

ipaH7.8p and rpsM

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSUM-GroEL-gfp

Plasmid

#109286

Purpose

Expresses gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GroEL-GFP

Expression

Bacterial

Mutation

GFP modified for expression in prokaryotes; inclu…

Promoter

GroEL

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX304-mNeonGreen

Plasmid

#162034

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Neon Green

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_B2520

Plasmid

#91077

Purpose

Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

OsU6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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AZ64_pE.DonorCLYBL.TS

Plasmid

#199228

Purpose

Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

Insert

Expression unit for EGFP reporter

Use

Gene targeting donor plasmid

Expression

Mammalian

Promoter

Human PGK1 gene promoter

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSMART-Ala1

Plasmid

#65814

Purpose

NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Insulated waaHp from E. coli

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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Cav1-GFP

Plasmid

#14433

Depositor

Ari Helenius

Article

Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

Insert

Caveolin-1 (CAV1 Canis familalis)

Tags

EGFP

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA

Plasmid

#235678

Purpose

Plasmid control for pcDNA3.1-HA-RAD52-WT and pcDNA3.1-HA-RAD52-∆C

Depositor

Simon Powell

Article

Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-ER-5

Plasmid

#55294

Purpose

Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

ER (CALR Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG2IG-Hmga1a

Plasmid

#122293

Purpose

Overexpression for mouse Hmga1a

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Hmga1a (Hmga1 Mouse)

Expression

Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pH2B-emiRFP703

Plasmid

#136567

Purpose

Histone 2B labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

H2B-emiRFP703

Tags

emiRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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H2B-mScarlet3-S2

Plasmid

#246872

Purpose

Expression of mScarlet3-S2 fused with H2B. This construct is used for imaging, photobleaching and photochromic behavior measurements.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Tags

H2B

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-UBE4B

Plasmid

#113499

Purpose

Expression of human UBE4B with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBE4B (UBE4B Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shLKB1-Hu

Plasmid

#61242

Purpose

Lentivirus expressing shRNA to human LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

LKB1 (STK11 Human)

Use

Lentiviral

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shLKB1-Ms

Plasmid

#61231

Purpose

Lentivirus expressing shRNA to mouse LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (Stk11 Mouse, Human)

Use

Lentiviral

Promoter

H1/TO

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pCAG_mRuby2_smFP FLAG

Plasmid

#59760

Purpose

encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

mRuby2_smFP_FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pemiRFP703-tubulin

Plasmid

#136564

Purpose

Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP703-tubulin

Tags

emiRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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