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pEBTetBl-CLIP-Cep131

Plasmid

#136875

Purpose

Mammalian expression of the centrosomal protein Cep131 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Publication

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep131 (CEP131 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep72

Plasmid

#136819

Purpose

Mammalian expression of the centrosomal protein Cep72 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Publication

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep72 (CEP72 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEBTet-SNAP-Cep57

Plasmid

#136817

Purpose

Mammalian expression of the centrosomal protein Cep57 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Publication

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep57 (CEP57 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC39A14

Plasmid

#132105

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

Insert

SLC39A14 (SLC39A14 Human)

Expression

Mammalian

Available since

Dec. 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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JL202: pMVP/mCherry-DEST

Plasmid

#121853

Purpose

pMVP destination vector, empty expression vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB121

Plasmid

#68575

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Promoter

MpEF1alpha promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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M3938 trp1::ADE2 Disruptor Converter

Plasmid

#51673

Purpose

Yeast marker swap plasmid for converting TRP1 to ADE2

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Promoter

ADE2

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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D588 lys2::HIS3 Disruptor Converter

Plasmid

#51675

Purpose

Yeast marker swap plasmid for converting LYS2 to HIS3

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

HIS3 (HIS3 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pQTEV-ILKAP

Plasmid

#34817

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

integrin-linked kinase-associated serine/threonine phosphatase 2C (ILKAP Human)

Tags

His and TEV

Expression

Bacterial

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hADM-RFP

Plasmid

#22922

Depositor

Edward Callaway

Publication

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human adrenomedullin promoter driving DsRedExpress (ADM Human)

Use

AAV

Expression

Mammalian

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-fTCAP-GFP

Plasmid

#22916

Depositor

Edward Callaway

Publication

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu titan cap promoter driving GFP

Use

AAV

Expression

Mammalian

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pSc-His6-Sem1Sc

Plasmid

#83235

Purpose

pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

Depositor

Gali Prag

Publication

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

Sem 1 (SEM1 Budding Yeast)

Tags

His6

Expression

Yeast

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB424

Plasmid

#68689

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Promoter

MpEF1alpha promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB414

Plasmid

#68679

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSFFV-Cx43

Plasmid

#69442

Purpose

mammalian expression of rat Cx43

Depositor

Richard Veenstra

Publication

Veenstra et al Circ Res. 1995 Dec;77(6):1156-65.

Insert

Cx43 (Gja1 Rat)

Expression

Mammalian

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
MR015-BAG3 Right TALEN

Plasmid

#49466

Purpose

Right TALEN for BAG3

Depositor

Bruce Conklin

Publication

Miyaoka et al Nat Methods. 2014 Mar;11(3):291-3. doi:

Insert

TALEN

Use

TALEN

Expression

Mammalian

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep78

Plasmid

#136827

Purpose

Mammalian expression of the centrosomal protein Cep78 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Publication

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep78 (CEP78 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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LB601: pMVP/PB/eGFP-DEST

Plasmid

#121870

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR)

Plasmid

#107137

Purpose

Dominant negative PKA cGAL effector for C. elegans

Depositor

Paul Sternberg

Publication

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

kin-2a(G310D)::SL2::GFP

Expression

Worm

Promoter

15xUAS-delta-pes-10(basal promoter)

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-fSFRP2-GFP

Plasmid

#22915

Depositor

Edward Callaway

Publication

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu secreted frizzled receptor protein 2 promoter driving GFP

Use

AAV

Expression

Mammalian

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only