We narrowed to 19,143 results for: REV

Showing: 11021 - 11040 of 19143 results

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pENTR-MBD1

Plasmid

#47057

Purpose

Expresses methyl-CpG binding domain protein 1

Depositor

Huda Zoghbi

Article

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Methyl-CpG Binding Domain Protein 1 (MBD1 Human)

Use

Gateway entry vector

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pCAGGS-Flag-hsDicer (D1320A/D1709A)

Plasmid

#41585

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

D1320A/D1709A

Promoter

CMV

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only
3543 pMIG Bcl-XL mut8

Plasmid

#8792

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bcl-XL (BCL2L1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

G138A, R139L, I140N

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
Ntn1-AP-His

Plasmid

#71978

Purpose

Expresses the entire Netrin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn1 (Ntn1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGG-NFIX2

Plasmid

#112699

Purpose

Expression of NFIX2 protein and GFP

Depositor

Richard Gronostajski, Francois Guillemot

Article

Martynoga et al Genes Dev. 2013 Aug 15;27(16):1769-86. d

Insert

Nuclear Factor I X2 (Nfix Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

Plasmid

#156463

Purpose

Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

Depositor

Andre Stiel

Article

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

Heme Oxygenase
ReBphP-PCM

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-TAOK2

Plasmid

#172992

Purpose

constitutive expression of FLAG-tagged TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-TAOK2 (TAOK2 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-HOIP-Strep-HA

Plasmid

#113494

Purpose

Expression of human HOIP with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

HOIP (RNF31 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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BRCA2 A12.2 gRNA

Plasmid

#90551

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA2 (Guide Designation A12.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pX330_TOMM20 sgRNA / hSpCas9

Plasmid

#172836

Purpose

Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.0-Pct5.1-crRNA(AarI)

Plasmid

#191633

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloning

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pYES260-sc.eEF2 K509A

Plasmid

#85120

Purpose

yeast expression of mutant EEF2

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFT1/EFT2 (EFT2, EFT1 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K509A

Promoter

GAL1

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-LGALS8

Plasmid

#175758

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS8 (LGALS8 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pPgiCas13b-gRNA-1

Plasmid

#184834

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPgiCas13b-gRNA-NT

Plasmid

#184835

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMy-MouseCTCFbiotag-T2A-mOrange

Plasmid

#50564

Purpose

Retroviral vector endoding for mOrange and mouse CTCF with a short biotinylation substrate (biotag, Kimetal.,2009) fused to its C-term

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Inserts

Ampicilin
Mouse CTCF Exons

Use

Retroviral

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-MFA-NFAPoptim

Plasmid

#221095

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p23-NES-PspCas13b-msfGFP-NES-Flag

Plasmid

#165071

Purpose

overexpression of PspCas13b in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

PspCas13b

Use

Lentiviral

Expression

Mammalian

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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Plxna4-Fc-His

Plasmid

#72125

Purpose

Expresses the extracellular region of the PlexinA4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna4 (Plxna4 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBXD3-Strep-HA

Plasmid

#113488

Purpose

Expression of human UBXD3 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBXD3 (UBXN10 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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