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PB-EF1-H4-HaloTag-IRES-Neo

Plasmid

#247450

Purpose

Expresses wild-type H4-Halo under EF1 promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-IB-H2B-PA-mCherry

Plasmid

#247339

Purpose

Expresses photoactivatable H2B-Cherry under CAG promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4(Q5,8,12,16)-HaloTag-FRT

Plasmid

#247454

Purpose

Expresses wild-type H4(Q5,8,12,16)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4(Q5,8,12,16) has four mutations in the N-termin…

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4Δ1-19-HaloTag-FRT

Plasmid

#247456

Purpose

Expresses wild-type H4(Δ1-19)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4Δ1-19 lacks the initial 19 residues at the N-te…

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4(R5,8,12,16)-HaloTag-FRT

Plasmid

#247453

Purpose

Expresses wild-type H4(R5,8,12,16)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4(R5,8,12,16) has four mutations in the N-termin…

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Nav1.6(K1425TAG)-HA

Plasmid

#239763

Purpose

Expresses mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Scn8a (Scn8a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

K1425TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Nav1.6(K1546TAG)-HA

Plasmid

#239764

Purpose

Expresses mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Scn8a (Scn8a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

K1546TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID-ER

Plasmid

#240230

Purpose

Expression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-sfGFP-TurboID-ER

Plasmid

#240232

Purpose

Expression of ER-localized sfGFP-TurboID under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Myc-BirA*G3-ER

Plasmid

#240234

Purpose

Expression of ER-localized Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

BiP-Myc-BirA*G3-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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