We narrowed to 1,251 results for: Lyn

1. pCMVtrunc Affimer6-sfCherry2ΔN12

   Plasmid

   #231551

   Purpose

   Mammalian expression of Affimer6 fused to N-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Affimer6
   

   Use

   Tags

   sfCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

   Plasmid

   #159107

   Purpose

   The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

   Depositor

   Thomas Tuschl

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   NSP12
   

   Use

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pnEA-vH_His-TEV-SEPT2_SEPT6

   Plasmid

   #174497

   Purpose

   bacterial co-expression of human SEPT2 and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a 6xHis PreScission SARS-CoV-2 nsp13

   Plasmid

   #159390

   Purpose

   Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

   Depositor

   Tarun Kapoor

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnCS_SEPT6_SEPT7-TEV-Strep

   Plasmid

   #174499

   Purpose

   bacterial co-expression of human SEPT6 and of human SEPT7

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN6 (SEPTIN6 Human)
   SEPTIN7 (SEPTIN7 Human)
   

   Use

   Tags

   TEV-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMVtrunc Affimer6-msfGFPΔN12

   Plasmid

   #231550

   Purpose

   Mammalian expression of Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Affimer6
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX_hDDX5_FL_wt

   Plasmid

   #88871

   Purpose

   inducible expression of GST-DDX5 in bacteria

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-NDUFA5-mVenus

   Plasmid

   #185646

   Purpose

   NDUFA5 Complex I sub-unit with C-terminus mVenus tag

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   NDUFA5 (NDUFA5 Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX_hDDX5_FL_D248N

   Plasmid

   #88872

   Purpose

   inducible expression of GST-DDX5 D248N in bacteria

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   D248N

   Promoter

   Tac

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-NDUFV2-mVenus

    Plasmid

    #185653

    Purpose

    NDUFV2 Complex I sub-unit with C-terminus mVenus tag

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    NDUFV2 (NDUFV2 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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