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pHRSV_CHARMS-17xTA

Plasmid

#233934

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-17xTAcontains 17 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-17xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-20xTA

Plasmid

#233935

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-10xTAcontains 20 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-20xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-TIR

Plasmid

#233919

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS): CHARMS-TIR

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-TIR

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDEHISSIRV55

Plasmid

#97055

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 55 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 55

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pUAST flag Rab23 WT

Plasmid

#53488

Depositor

Matthew Scott

Insert

Rab23 (Rab23 Fly)

Tags

flag

Expression

Insect

Promoter

GAL4

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pUAST myc Rab23 WT

Plasmid

#53486

Depositor

Matthew Scott

Insert

Rab23 (Rab23 Fly)

Tags

myc

Expression

Insect

Promoter

GAL4

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
p2OT-MtbTOP1

Plasmid

#250351

Purpose

Expresses Mycobacterium tuberculosis topoisomerase I protein with N-terminal His6-Mocr tag

Depositor

Yuk-Ching Tse-Dinh

Article

Tan et al J Mol Biol. 2016 Jan 16;428(1):182-193.

Insert

Mycobacterium tuberculosis topoisomerase I

Tags

6xHis mocr

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pDEHISSIRV119

Plasmid

#97054

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 119 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 119

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEHISSSO1986

Plasmid

#97057

Purpose

Expressing Sulfolobus solfataricus orf SSO1986 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO1986

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-bDD-5xT6BMs

Plasmid

#233939

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-bDD with 5 TRAF6 binding motifs

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-bDD-5xT6BMs

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-17xTA-3xAla

Plasmid

#233936

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-17xTA with alanine mutation in the TRAF6 binding motif

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-17xTA-3xA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-20xTA-3xAla

Plasmid

#233937

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-20xTA with alanine mutation in the TRAF6 binding motif

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-20xTA-20xA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-TIR-5xT6BMs

Plasmid

#233938

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-TIR with 5 TRAF6 binding motifs

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-TIR-5xT6BMs

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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p2705-AGER-eGFP

Plasmid

#216474

Purpose

donor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

eGFP

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLT3REVIR_MARK3 2573

Plasmid

#107233

Purpose

shRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3 shRNA (22 nt) (MARK3 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLT3REVIR_MARK3 1122

Plasmid

#107232

Purpose

shRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3 shRNA (22 nt) (MARK3 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pEAHISPA5006

Plasmid

#97016

Purpose

Expressing Pseudomonas aeruginosa orf PA5006 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

P aeruginosa orf PA5006

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSATAG

Plasmid

#97004

Purpose

Expressing MRSA TAG protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

DNA-3-methyladenine glycosylase I

Tags

N-ter TEV protease cleavable 6His

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV CAG Nuc-flox(mCherry)-EGFP

Plasmid

#256094

Purpose

Express a Cre-dependent mCherry to EGFP Fluorescent protein

Depositor

Baoji Xu

Article

Niu et al Biomolecules. 2024 Jan 11;14(1):91. doi:

Insert

CAG (EF1a promoter in the backbone was replaced by CAG promoter)

Use

AAV

Tags

None

Promoter

CAG

Available Since

June 8, 2026

Availability

Academic Institutions and Nonprofits only

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p2OT-MtbTOP1-704t

Plasmid

#250352

Purpose

Expresses truncated Mycobacterium tuberculosis topoisomerase I with N-terminal His6-Mocr tag

Depositor

Yuk-Ching Tse-Dinh

Article

Tan et al J Mol Biol. 2016 Jan 16;428(1):182-193.

Insert

Mycobacterium tuberculosis topoisomerase I residues 2-704

Tags

6xHis mocr

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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