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pMRMTK-clo28

Plasmid

#204002

Purpose

Cloning plasmid containing the Bpi ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Publication

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Bpi ES3

Expression

Plant

Promoter

Bpi ES3

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo1

Plasmid

#203919

Purpose

Cloning plasmid containing the BHR1 origin insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Publication

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

BHR1 Origin of Replication

Expression

Plant

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET-GFP-mSOX17

Plasmid

#206379

Purpose

Expresses EGFP fused mouse SOX17 in bacteria, for protein production.

Depositor

Ralf Jauch

Publication

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17 (Sox17 Mouse)

Tags

EGFP

Expression

Bacterial

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Im7 F84A

Plasmid

#201082

Purpose

Im7 F84A variant inserted into CysGA at G364 via GS linker

Depositor

Shu Quan

Publication

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Im7 F84A

Expression

Bacterial

Mutation

Im7 F84A

Promoter

pTrc

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET-GFP-mSOX17FNV

Plasmid

#206380

Purpose

Expresses EGFP fused mouse SOX17FNV in bacteria, for protein production.

Depositor

Ralf Jauch

Publication

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV (Sox17 Mouse)

Tags

EGFP

Expression

Bacterial

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET-GFP-miniSOX

Plasmid

#206381

Purpose

Expresses EGFP fused miniSOX in bacteria, for protein production.

Depositor

Ralf Jauch

Publication

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

miniSOX

Tags

EGFP

Expression

Bacterial

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSuperior.retro.puro-sh-γ-tubulin

Plasmid

#205602

Purpose

Tet-inducible expression of shRNA targeting gamma-tubulin in human cells, retroviral

Depositor

Tarun Kapoor

Publication

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

sh-g-Tubulin

Expression

Mammalian

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNMSB88

Plasmid

#199321

Purpose

Combine with Gal4 to direct TeNL expression

Depositor

Michael Krieg

Publication

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

15xUAS::delta pes-10::::TeNL::let-858 3'UTR

Expression

Worm

Mutation

KD for calcium is 250 nM, 1 synthetic intron

Promoter

15xUAS::delta pes-10

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pNMSB109

Plasmid

#199322

Purpose

Combine with Gal4 to direct CaMBI 300 expression

Depositor

Michael Krieg

Publication

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

15xUAS::delta pes-10::::CaMBI::let-858 3'UTR

Expression

Worm

Mutation

Orange CaMBI with 300 nM KD for calcium

Promoter

15xUAS:::delta pes-10

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T100-Cterminal-mEmerald

Plasmid

#202352

Purpose

Talin(FullLength)-mEmerald

Depositor

Pakorn Kanchanawong

Publication

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(FullLength)-mEmerald

Expression

Mammalian

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-TcCas12b-T2A-EGFP

Plasmid

#188277

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Publication

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

TcCas12b

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE Myc-epitope donor ORF2

Plasmid

#200376

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Publication

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Expression

Mammalian

Mutation

NA

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_B-Pho2-1/2-2-Csy4

Plasmid

#161760

Purpose

MODULE B Csy4 vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2103 - #91061)

Depositor

Shaun Curtin

Publication

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

gRNAs

Expression

Bacterial

Promoter

CmYLCV Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H05R

Plasmid

#198674

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Publication

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI3_DW

Use

Synthetic Biology

Expression

Yeast

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H05L

Plasmid

#198673

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Publication

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI3_UP

Use

Synthetic Biology

Expression

Yeast

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGA-red-mini

Plasmid

#196338

Purpose

Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

Depositor

Paola Branduardi

Publication

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Golden gate assembly backbone

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-PhiC31-9

Plasmid

#197725

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

PhiC31 recombinase (tomato optimized)

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-ELuc-9

Plasmid

#197736

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Emerald/Enhanced Click Beetle Luciferase (ELuc) (tomato codon-optimized)

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPINA7A2mTFP1(F)

Plasmid

#193774

Purpose

Expresses mTFP1 under the control of PINA7 synthetic promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Publication

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

mTFP1

Use

Synthetic Biology

Expression

Bacterial

Promoter

PINA7 synthetic promoter carrying binding sites f…

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1050T

Plasmid

#191374

Purpose

pBac-AedesUbiq-RfxCas13d-p10-Opie2-dsRed.

Depositor

Omar Akbari

Publication

Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

Insert

Uniq-CasRx

Expression

Insect

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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