We narrowed to 12,753 results for: CAR

1. pDD2711

   Plasmid

   #175083

   Purpose

   Mammalian expression of Flag-tagged KSHV ORF45 carrying E223A-S226A/RC mutations

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

   Insert

   KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E223A, S226A, RC mutations

   Promoter

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD2712

   Plasmid

   #175084

   Purpose

   Mammalian expression of Flag tagged KSHV ORF45 carrying E223A-S226A/RN2 mutations

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

   Insert

   KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E223A, S226A, RN2 mutations

   Promoter

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS42K_MCP-VP64_D-MS2

   Plasmid

   #177161

   Purpose

   Expresses transactivator (MCP-VP64) and scRNA (D-MS2) in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Inserts

   MCP-VP64
   D-MS2
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter and SNR52 promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS42K_PCP-VP64_A-PP7

   Plasmid

   #177156

   Purpose

   Expresses transactivator (PCP-VP64) and scRNA (A-PP7) in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Inserts

   PCP-VP64
   A-PP7
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter and SNR52 promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS42K_MCP-VP64_C-MS2

   Plasmid

   #177160

   Purpose

   Expresses transactivator (MCP-VP64) and scRNA (C-MS2) in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Inserts

   MCP-VP64
   C-MS2
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter and SNR52 promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pStr-KDEL_APV-SBP-Dendra2-ER-5

   Plasmid

   #170679

   Purpose

   Expresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Inserts

   APV-SBP-Dendra2-ER-5
   streptavidin-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   IRES and T7

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS105

   Plasmid

   #174830

   Purpose

   Encodes a unique yeast telomere end at TEL11R by removing the subtelomere

   Depositor

   Carol Greider

   Article

   Sholes et al Genome Res. 2022 Apr;32(4):616-628. doi:

   Insert

   11R Unique
   

   Use

   Tags

   GAL4 5XUAS

   Expression

   Bacterial

   Mutation

   Changing TEL11R to a unique end

   Promoter

   lac promoter

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. L1_NOP1:mS-N7-Ck2

   Plasmid

   #136126

   Purpose

   L1 in position 2, example of weak promoter

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpNOP1:mScarletI-N7_3tNos-35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. OfCasp3a

   Plasmid

   #166053

   Purpose

   a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspase

   Depositor

   Clay Clark

   Article

   Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

   Insert

   Orbicella faveolata caspase-3a
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lac promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PaCasp7a

    Plasmid

    #166051

    Purpose

    a caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspase

    Depositor

    Clay Clark

    Article

    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

    Insert

    Porites astreiodes caspase-7a
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. IBMc120

    Plasmid

    #161950

    Purpose

    Contains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB4A3-PECF20-B30-mScarlet-I-B15
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PECF20

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. IBMc393

    Plasmid

    #161953

    Purpose

    Demonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB4A3-T-PT7-B30-mScarlet-I-3T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. IBMc330

    Plasmid

    #161952

    Purpose

    Contains elements for building reporter plasmid for IBM. SrpR reporter plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSEVA171-PSrpR-B30-mScarlet-I-Ter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PSrpR

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. IBMc050

    Plasmid

    #161938

    Purpose

    Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plux2

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. IBMc259

    Plasmid

    #161951

    Purpose

    Contains elements for building reporter plasmid for IBM.TetR reporter plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PTet*

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Nup54 no FG-mEGFP494(2)

    Plasmid

    #164271

    Purpose

    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFP

    Depositor

    Sanford Simon

    Article

    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

    Insert

    Nup54 (NUP54 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    deleted the FG region of Nup54

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Nup54 no FG-mEGFP494(1)

    Plasmid

    #164270

    Purpose

    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFP

    Depositor

    Sanford Simon

    Article

    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

    Insert

    Nup54 (NUP54 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    deleted the FG region of Nup54

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXW101Plux2-Pecf11-gfp

    Plasmid

    #160823

    Purpose

    Two-layered AHL-responsive circuit carrying an ECF11-based amplifier

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

    Insert

    PJ23101-30luxR-t-Plux2-33ecf11-Pecf11-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23101, Plux2, Pecf11

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQadd1F

    Plasmid

    #135660

    Purpose

    Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site.

    Depositor

    Andrew Tolonen

    Article

    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

    Insert

    Intron containing lox site
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pFer

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQadd1R

    Plasmid

    #135661

    Purpose

    Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location.

    Depositor

    Andrew Tolonen

    Article

    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

    Insert

    Intron containing lox site
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pFer

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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