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pmcsg7-mCRT(D311A)

Plasmid

#83509

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Biol Chem. 2011 Mar 18;286(11):8771-85

Insert

CALR (Calr Mouse)

Tags

His

Expression

Bacterial

Mutation

D311A

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pmcsg7-mCRT(1-351)

Plasmid

#83508

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Biol Chem. 2011 Mar 18;286(11):8771-85

Insert

CALR (Calr Mouse)

Tags

His

Expression

Bacterial

Mutation

1-351 only

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pILGFPH0

Plasmid

#83552

Purpose

Used to evaluate the expression output of TPS1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TPS1 promoter-yEGFP

Expression

Yeast

Promoter

TPS1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP90

Plasmid

#83547

Purpose

Used to evaluate the expression output of TEF2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF2 promoter-yEGFP

Expression

Yeast

Promoter

TEF2

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP82

Plasmid

#83541

Purpose

Used to evaluate the expression output of YEF3 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

YEF3 promoter-yEGFP

Expression

Yeast

Promoter

YEF3

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP80

Plasmid

#83540

Purpose

Used to evaluate the expression output of RPL3promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL3 promoter-yEGFP

Expression

Yeast

Promoter

RPL3

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP78

Plasmid

#83539

Purpose

Used to evaluate the expression output of SSB1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSB1 promoter-yEGFP

Expression

Yeast

Promoter

SSB1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCas59

Plasmid

#82396

Purpose

sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCas57

Plasmid

#82395

Purpose

sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas56

Plasmid

#82394

Purpose

sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas55

Plasmid

#82393

Purpose

sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas52

Plasmid

#82392

Purpose

sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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GST-PRMT9 deltaC 350 (stop)

Plasmid

#67612

Purpose

bacterial expression of GST-PRMT9 missing residues 351-845

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

Stop codons placed at aa 351 and 352 to create C …

Promoter

tac

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only
pSFFV-Cx45

Plasmid

#69808

Purpose

mammalian expression of chick Cx45

Depositor

Richard Veenstra

Article

Veenstra et al Circ Res. 1992 Nov;71(5):1277-83.

Insert

Cx45 (GJC1 Chicken)

Expression

Mammalian

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
pDEST14-ARF4

Plasmid

#67371

Purpose

Expression of native hArf4 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCS-flt4um159-HA

Plasmid

#67704

Purpose

Expression construct

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

flt4 (flt4 Zebrafish)

Tags

HA

Expression

Mammalian

Mutation

um159

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCS-Fv2Eflt4um200-2a-tdTomato

Plasmid

#67698

Purpose

Expression construct

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4-2A-tdTomato (flt4 Zebrafish)

Tags

2A-tdTomato and HA

Mutation

um200

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only
CFP(1-158) in pcDNAI/Amp

Plasmid

#60895

Purpose

This vector attaches CFP(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

CFP(1-158)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pRevTre-mKLF5

Plasmid

#41744

Depositor

Jonathan Katz

Article

Yang et al Cell Cycle. 2012 Nov 1;11(21):4033-9. do

Insert

Klf5 (Klf5 Mouse)

Expression

Mammalian

Available Since

July 13, 2015

Availability

Academic Institutions and Nonprofits only
pCNL-N1_Golgi

Plasmid

#65694

Purpose

Expresses Golgi-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

N-terminal 81 amino acids of beta 1,4-galactosyltransferase

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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