We narrowed to 19,435 results for: Kin
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Plasmid#55200PurposePlasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.DepositorInsertdsRed2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mGM-CSF
Plasmid#74465Purposeexpression of mGM-CSF in mammalian cellsDepositorInsertgranulocyte-monocyte colony-stimulating factor (Csf2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sox2-CreERT2
Plasmid#135618PurposeDonor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locusDepositorInsertP2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsP2A-CreERT2ExpressionMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2
Plasmid#69623PurposeA FRET sensor for Galphai1 activation, encoded on a single plasmid.DepositorInsertsGbeta-2A-cpV-Ggamma2
Galphai-mTurquoise2
UseTagsciruclar permutated Venus (cp173V) and mTurquoise…ExpressionMammalianMutationPromoterCMV and CMV (IRES)Available sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
PR_GEF (2XPDZ-mCherry-Larg (DH)
Plasmid#80407PurposeRecruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cellsDepositorInsert2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)
UseTagsmCherryExpressionMammalianMutationDH domain only (aa 766-997)PromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE
Plasmid#196677PurposeAAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).DepositorInsertPKIα-P2A-mRuby3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Ef1a:PE2-P2A-GFP
Plasmid#184445PurposeSingle EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and Ef1AAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash5
Plasmid#74133PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HLA-Flag-natT1R3
Plasmid#113950Purposemammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R3 (TAS1R3 Human)
UseTagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationresidues 21-852PromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-ch-TOG-His6
Plasmid#69108PurposeFor bacterial expression of recombinant human ch-TOG tagged with MBP and His.DepositorInsertColonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3−3xFLAG−AGO2(D669A)
Plasmid#220461PurposeExpress catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cellsDepositorInsertHuman AGO2 (AGO2 Human)
UseTags3xFLAGExpressionMammalianMutationAspartate 669 to alaninePromoterCMVAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK2 wt
Plasmid#80870Purposemammalian expression of ALK2 WTDepositorInsertALK2 (ACVR1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9-Cdt1
Plasmid#80426PurposeUsed for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.DepositorInsertSpCas9-Cdt1
UseCRISPRTagsCdt1ExpressionMutationcodon optimized for humanPromoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBMN mEGFP-OPTN
Plasmid#188784PurposeFor mammalian expression of OPTN tagged with mEGFPDepositorInsertOPTN (OPTN Human)
UseRetroviralTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-Junb
Plasmid#178441Purposedoxycycline-inducible expression of Mouse Junb in mammalian cellsDepositorInsertJunb (Junb Mouse)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV CMV-DIO-(mCherry-U6)-shRNA(scrambled)
Plasmid#214733PurposeExpresses mCherry protein in infected and cre positive cellsDepositorInsertanti-Crh shRNA (scrambled) / mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas9(D10A)
Plasmid#74495PurposeNicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10ADepositorInsertCas9 D10A
UseSynthetic BiologyTagsExpressionBacterialMutationD10APromoterpTetoAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEP-LAMP1-RFP
Plasmid#167970PurposeExpresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFPDepositorInsertLAMP1 (Lamp1 Rat)
UseTagsSEP and mRFPExpressionMammalianMutationD50EPromoterCMVAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2
Plasmid#219548PurposeNLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.DepositorInsertNLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
UseTagsExpressionMammalianMutationThe (SAGG)x4 linker between GAL4(1-65) and the PC…PromoterCMVd1Available sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-OTX2-P2A-mCherry BlastR
Plasmid#186741PurposeDox inducible OTX2-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertOTX2 (OTX2 Human)
UseLentiviralTagsP2A-mCherryExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only