We narrowed to 15,769 results for: LEA

1. 6CUI (BRD2)

   Plasmid

   #119963

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   BRD2 (BRD2 Human)
   

   Tags

   GST tag followed by a long linker and TEV cleavag…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 1, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. 6DUB (LOC149281)

   Plasmid

   #119967

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   LOC149281 (METTL11B Human)
   

   Tags

   6xHIS, thrombin,SUMO and TEV cleavage

   Expression

   Bacterial

   Mutation

   contains amino acids 58-278

   Promoter

   T7

   Available Since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pMLS415

   Plasmid

   #114272

   Purpose

   SapTrap donor vector for point mutagenesis; pENTR-221::syntron(lox2272-119+)

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

   Insert

   Cbr-Unc-119
   

   Use

   CRISPR and Cre/Lox

   Mutation

   *please see depositor comment below

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Myc-gamma1 (in pCAGGS)

   Plasmid

   #118039

   Purpose

   GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG1 (Gabrg1 Rat)
   

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. FOXN1 (FOXN1A-c017)

   Plasmid

   #110249

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   FOXN1 (FOXN1 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7 - lacO

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. LIMK2 (LIMK2A-c052)

   Plasmid

   #110264

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   LIMK2 (LIMK2 Human)
   

   Tags

   His-TEV

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJExpress414_GmPOPB-R663K

   Plasmid

   #92239

   Purpose

   Express R663K mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-R663K mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Arginine 663 to Lysine

   Promoter

   T7 promoter

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2QO2 (EPHA3)

   Plasmid

   #101271

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EPHA3 (EPHA3 Human)
   

   Tags

   Hexahistidine tag with Thrombin cleavage

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 2, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. IPCEF1 (PIP3EA-c002)

   Plasmid

   #98240

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PIP3EA (IPCEF1 Human)
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJExpress414_GmPOPB-W695del

    Plasmid

    #92241

    Purpose

    Express W695-deletion mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-W695deletion mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Tryptophan 695 deleted

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJExpress414_GmPOPB-R663Q

    Plasmid

    #92240

    Purpose

    Express R663Q mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-R663Q mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Arginine 663 to Glutamine

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJExpress414_GmPOPB-H698A

    Plasmid

    #92237

    Purpose

    Express H698A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-H698A mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Histidine 698 to Alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJExpress414_GmPOPB-R663A

    Plasmid

    #92238

    Purpose

    Express R663A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-R663A mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Arginine 663 to Alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEHIShqprtK139A

    Plasmid

    #92403

    Purpose

    hQPRTase harbouring K139A mutation

    Depositor

    Huanting Liu, James Naismith

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Tags

    TEV cleacable 6xHis

    Expression

    Bacterial

    Mutation

    K139A

    Promoter

    T7

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a - TryX

    Plasmid

    #89623

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Tryparedoxin
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR-IAA8

    Plasmid

    #83873

    Purpose

    Gateway donor vector for IAA8

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA8 (IAA8 Mustard Weed)
    

    Use

    Gateway donor vector

    Available Since

    Oct. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR-IAA16

    Plasmid

    #83854

    Purpose

    Gateway donor vector for IAA16

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA16 (IAA16 Mustard Weed)
    

    Use

    Gateway donor vector

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGE63

    Plasmid

    #79445

    Purpose

    recipient plasmid [single nuclease]; p35S:Cas9, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. 4QL1

    Plasmid

    #61942

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5:APC041-G05:C36415 (WDR5 Human)
    

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. GMIP

    Plasmid

    #32046

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GMIP (GMIP Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 80-357 only

    Available Since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits