We narrowed to 15,769 results for: LEA

1. mScarlet-I-LaminB

   Plasmid

   #98831

   Purpose

   In vivo visualization of the nuclear envelope (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   CytERM
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   CMV

   Available Since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-GINKO1

   Plasmid

   #113111

   Purpose

   Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   GINKO1
   

   Tags

   6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmScarlet_NES_C1

   Plasmid

   #85060

   Purpose

   In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   NES
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBOB-synP-HTB

   Plasmid

   #30195

   Depositor

   Edward Callaway, Liqun Luo

   Article

   Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

   Insert

   histone-GFP-2A-TVA receptor-2A-rabies glycoprotein
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   April 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. SBE-NLS-mCherry PGK-rtTA3

   Plasmid

   #195332

   Purpose

   Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Inserts

   NLS-mCherry
   rtTA3
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   hPGK and miniCMV

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

   Plasmid

   #175547

   Purpose

   Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag7 and Tomm20-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-H-Luc_nucl

   Plasmid

   #113931

   Purpose

   Plasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   H-Luc_nucl
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS

   Plasmid

   #230058

   Purpose

   Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA1 single chain variable fragment (scFv)
   

   Tags

   6X HIS, HRV 3C PreScission protease, HaloTag, and…

   Expression

   Mammalian

   Promoter

   Chicken beta actin (CAG)

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS

   Plasmid

   #230059

   Purpose

   Expresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA2 single chain variable fragment (scFv)
   

   Tags

   6X HIS, HRV 3C PreScission protease, HaloTag, and…

   Expression

   Mammalian

   Promoter

   Chicken beta actin (CAG)

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. HTATIP (2OU2)

    Plasmid

    #33338

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HTATIP (KAT5 Human)
    

    Tags

    Hexahistidine tag with Thrombin cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 11, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

11. AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

    Plasmid

    #228364

    Purpose

    mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Enascas12a nuclease and en…

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMF-TetTight

    Plasmid

    #193288

    Purpose

    Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

    Depositor

    Rudolf Jaenisch

    Article

    Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

    Insert

    rtTA-P2A-tTS
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF‐1α

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLENTI_RPS10

    Plasmid

    #127129

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS10 (RPS10 Human)
    

    Use

    Lentiviral

    Mutation

    K24R- please see depositor comments

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. H2B-2A-GFP-IRESpuro2

    Plasmid

    #183746

    Purpose

    Expresses H2B-2A-GFP

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    H2B (H2BC16P Human)
    

    Tags

    GFP with 2A protease cleavage site between H2B an…

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SENP2M497A

    Plasmid

    #61088

    Purpose

    cleave the SUMO off when use PETSUMO vector for express fusion protein

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

    Insert

    SENP2M497A catalytic domain (SENP2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    M497A; Catalytic domain is residues 364-589

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-CAG-H2B-GFP

    Plasmid

    #116869

    Purpose

    Nuclear localized GFP

    Depositor

    Loren Looger

    Article

    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

    Insert

    GFP
    

    Use

    AAV

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b PKA Cat

    Plasmid

    #14921

    Depositor

    Susan Taylor

    Article

    Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

    Insert

    PKA catalytic subunit alpha (Prkaca Mouse)
    

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Available Since

    July 24, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTC217

    Plasmid

    #70018

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. mTurquoise2-LaminB

    Plasmid

    #98830

    Purpose

    In vivo visualization of the nuclear envelope (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    LaminB
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CMV

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDC191

    Plasmid

    #115792

    Purpose

    Expresses Rat synaptobrevin 2

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    Synaptobrevin2 (Vamp2 Rat)
    

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    S28C and C103S (please see comments below)

    Promoter

    T7

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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