We narrowed to 60,323 results for: cloning
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Plasmid#223192PurposeEntry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)DepositorInsertHAND1 (HAND1 Human)
UsePromoterless entry vector for gateway cloningTagsFLAG and T2A-mTagBFP2ExpressionBacterialPromoterNoneAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDonor-FMR1-CGG-BsmBI
Plasmid#222966Purpose0CGG with BsmBI site for inserting CGG repeatsDepositorInsertFMR1 homologous template (no CGG repeats) with cloning site (FMR1 Human)
UseCRISPRMutationCGG repeats deleted from 5' UTR, BsmBI site …Available SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-859
Plasmid#218009PurposemCerulean fluorescent protein fused to Factor Xa Protease cleavage site and Strep-Tag IIDepositorInsertmCerulean
UseSynthetic BiologyTagsFactor Xa protease cleavage site and Strep-tag IIAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pMBS-864
Plasmid#218018PurposeGST-tag fused to TEV Protease cleavage site for protein purification and cleavageDepositorInsertGST-tag
UseSynthetic BiologyTagsTEV protease cleavage siteAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-839
Plasmid#217995PurposesAP1 protein secretion signalDepositorInsertSecretion signal peptide derived from the secretory protein secreted acid phosphatase 1 (sAP1)
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-845
Plasmid#218000PurposeStrep-Tag II fused to PreScission Protease cleavage site for protein purification and cleavageDepositorInsertStrep-tag II
UseSynthetic BiologyTagsPreScission protease cleavage siteAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-848
Plasmid#218003PurposeGST-tag fused to PreScission Protease cleavage site for protein purification and cleavageDepositorInsertGST-tag
UseSynthetic BiologyTagsPreScission protease cleavage siteAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-849
Plasmid#218004Purpose8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavageDepositorInsert8xHis-tag
UseSynthetic BiologyTagsPreScission protease cleavage siteAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pDONR221-eGFP_myr
Plasmid#221412PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInserteGFP_myr
ExpressionMammalianAvailable SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-eGFP
Plasmid#221413PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInserteGFP
ExpressionMammalianAvailable SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-TMEM144
Plasmid#221426PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertTMEM144 (TMEM144 Human)
ExpressionMammalianAvailable SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Sensor_P2A_SLC2A4
Plasmid#221429PurposeGateway entry clone encoding a polyprotein that is cleaved to FRET glucose sensor and SLC2A4 proteinsDepositorInsertSensor_P2A_SLC2A4 (SLC2A4 Synthetic)
ExpressionMammalianAvailable SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA320
Plasmid#215952PurposeFragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surfaceDepositorHas ServiceCloning Grade DNAInsertrev{U6_v1; DR_v0 [EnAs]; sgCD47_v1 [EnAs]; DR_v1 [EnAs]; sgCD47_v2 [EnAs]}
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA315
Plasmid#215951PurposeFragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethalDepositorHas ServiceCloning Grade DNAInsertrev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA324
Plasmid#215953PurposeFragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surfaceDepositorHas ServiceCloning Grade DNAInsertrev{U6_v1; DR_v0[EnAs]; sgCD46_v4[EnAs]; DR_v1[EnAs]; sgCD47_v2[EnAs]; DR_v2[EnAs]; sgCD55_v4[EnAs];DR_v3[EnAs];sgCD81_v4[EnAs]}
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA074
Plasmid#215933PurposeFragmid fragment: (guide cassette) Tet-regulatable guide expression; positive control cell surfaceDepositorHas ServiceCloning Grade DNAInsertH1-2xTetO_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA435
Plasmid#215962PurposeFragmid fragment: (guide cassette) guide expression; positive control cell surfaceDepositorHas ServiceCloning Grade DNAInsertU6_v1; DR_v0 [EnAs]; sgCD274_v1; DR_v1 [EnAs]; sgADGRE5_v3; DR_v2; sgCD4_v1; DR_v3; sgDPP4_v1
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only