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Bo_NPTII:pKST:Gus_introns

Plasmid

#215175

Purpose

Evaluating guard cell specific promotors in Brassica

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

Inwardly rectifying K+ channel1

Use

Synthetic Biology

Promoter

pKST1

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAL008

Plasmid

#233849

Purpose

MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NLS-NS3-V1-VP48-2XOaf1-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-LexA-NLS-NS3-V1-VP48-2XOaf1-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN021

Plasmid

#233851

Purpose

MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NLS-NS3-V2-VP48-2XOaf1-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-LexA-NLS-NS3-V2-VP48-2XOaf1-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-TMEM9-EGFP

Plasmid

#225515

Purpose

Lentiviral TMEM9-EGFP expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM9 (TMEM9 Human)

Use

Lentiviral

Tags

EGFP

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHK001.3QS

Plasmid

#235485

Purpose

inducible NOT gate receiver with bs for sgRNA3

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK001.23

Plasmid

#235484

Purpose

dual NOT gate receiver with bs for sgRNA2 and sgRNA3

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-A*24:02

Plasmid

#235133

Purpose

E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*24:02 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pOP795

Plasmid

#235700

Purpose

Protein expression His-taged HsNRBF2_FL in bacteria

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

NRBF2 (NRBF2 Human)

Tags

His6(TEV)

Expression

Bacterial

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C233

Plasmid

#235629

Purpose

Encodes ALIBi C-terminal fragment, residues 233-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 233-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C141

Plasmid

#235621

Purpose

Encodes ALIBi C-terminal fragment, residues 141-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 141-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C222

Plasmid

#235627

Purpose

Encodes ALIBi C-terminal fragment, residues 222-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 222-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C196

Plasmid

#235625

Purpose

Encodes ALIBi C-terminal fragment, residues 196-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 196-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C168

Plasmid

#235623

Purpose

Encodes ALIBi C-terminal fragment, residues 168-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 168-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C100

Plasmid

#235619

Purpose

Encodes ALIBi C-terminal fragment, residues 100-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 100-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagAF_C257

Plasmid

#235631

Purpose

Encodes ALIBi C-terminal fragment, residues 257-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 257-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051d_sgCiPELO #2

Plasmid

#229018

Purpose

Expression of CRISPRi PELO sgRNA #2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO (PELO Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)

Plasmid

#233873

Purpose

Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Tags

His

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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DEXsynth:ccdb-3xHA + RUBY

Plasmid

#233319

Purpose

GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and RUBY marker

Depositor

Thomas Baum

Insert

DEXsynth:ccdb-3xHA + RUBY

Use

Gateway Cloning

Tags

3xHA

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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Ubi:mTb-ccdb + mcherry

Plasmid

#233304

Purpose

GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of miniTurbo-ID paired with ccdb selection toxin and mCherry marker

Depositor

Thomas Baum

Insert

Ubi:mTb-ccdb + mcherry

Use

Gateway Cloning

Tags

miniTurbo-ID

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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DEXsynth:eGFP-ccdb + RUBY

Plasmid

#233316

Purpose

GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker

Depositor

Thomas Baum

Insert

DEXsynth:eGFP-ccdb + RUBY

Use

Gateway Cloning

Tags

eGFP

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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