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pMXs-uORFx2-mNgn2x3HA-P2A-mRuby2

Plasmid

#233166

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-uORFx3-mNgn2x3HA-P2A-mRuby2

Plasmid

#233167

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.23-WIPI1promotor

Plasmid

#206840

Purpose

The plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).

Depositor

Tassula Proikas-Cezanne

Article

Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

Insert

WIPI1 promotor (partial)

Use

Luciferase

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA GPR137

Plasmid

#226809

Purpose

Gateway entry vector for GPR137

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR137 (GPR137 Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_001164351

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S724A

Plasmid

#224735

Purpose

For expression of HA-tagged GTSE1 protein with S724A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 724 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S734A

Plasmid

#224736

Purpose

For expression of HA-tagged GTSE1 protein with S734A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 734 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S171A

Plasmid

#224723

Purpose

For expression of HA-tagged GTSE1 protein with S171A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 171 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S187A

Plasmid

#224724

Purpose

For expression of HA-tagged GTSE1 protein with S187A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 187 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S262A

Plasmid

#224725

Purpose

For expression of HA-tagged GTSE1 protein with S262A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 262 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S345A

Plasmid

#224726

Purpose

For expression of HA-tagged GTSE1 protein with S345A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 345 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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