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RS17055_pMQ30_KO_Kan

Plasmid

#185398

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS17055 upstream flanking homology region
RR42_RS17055 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)

Plasmid

#203542

Purpose

Make FIP200 NTD:ATG13:ULK1 complex

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

FIP200 (RB1CC1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-mNeonC

Plasmid

#178461

Purpose

The mNeonGreen fluorescent protein with an N-terminal 6xHis and a C-terminal free-Cysteine in pET28a

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen

Tags

6xHis

Expression

Bacterial

Mutation

Free cysteine at the C-terminus

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2572

Plasmid

#195683

Purpose

Expression/purification of 6xHis-TEV-MlaC(V171R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(V171R)

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1223

Plasmid

#195688

Purpose

Expression/purification of 6xHis-TEV-MlaDdelta173-183

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaDdelta173-183

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1909

Plasmid

#195690

Purpose

Expression/purification of 6xHis-TEV-MlaD(F118A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaD(F118A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1910

Plasmid

#195691

Purpose

Expression/purification of 6xHis-TEV-MlaD(L123A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaD(L123A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2555

Plasmid

#195673

Purpose

Expression/purification of 6xHis-TEV-MlaC(G170K)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(G170K)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2556

Plasmid

#195674

Purpose

Expression/purification of 6xHis-TEV-MlaC(I130D)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(I130D)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2559

Plasmid

#195675

Purpose

Expression/purification of 6xHis-TEV-MlaC(L76D)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(L76D)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2560

Plasmid

#195676

Purpose

Expression/purification of 6xHis-TEV-MlaC(N155A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(N155A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2561

Plasmid

#195677

Purpose

Expression/purification of 6xHis-TEV-MlaC(N179E)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(N179E)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2563

Plasmid

#195678

Purpose

Expression/purification of 6xHis-TEV-MlaC(R134E)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(R134E)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2564

Plasmid

#195679

Purpose

Expression/purification of 6xHis-TEV-MlaC(R147D)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(R147D)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2565

Plasmid

#195680

Purpose

Expression/purification of 6xHis-TEV-MlaC(R153A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(R153A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2566

Plasmid

#195681

Purpose

Expression/purification of 6xHis-TEV-MlaC(R186A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(R186A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2568

Plasmid

#195682

Purpose

Expression/purification of 6xHis-TEV-MlaC(T176R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(T176R)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2573

Plasmid

#195684

Purpose

Expression/purification of 6xHis-TEV-MlaC(Y72A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(Y72A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2574

Plasmid

#195685

Purpose

Expression/purification of 6xHis-TEV-MlaC(Y82K)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(Y82K)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2576

Plasmid

#195686

Purpose

Expression/purification of 6xHis-TEV-MlaC(Y164R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(Y164R)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge