Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 23,473 results for: SCR

Showing: 11401 - 11420 of 23473 results

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lenti-Cas9-sgHPRT1

Plasmid

#196713

Purpose

CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Insert

Cas9-T2A-BSD-U6-sgHPRT1

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

EF1a/hU6

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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ptRNAfMet-A37U

Plasmid

#196659

Purpose

Plasmid for overexpression of mutated initiator tRNA (A37U)

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

tRNAfMet-A37U expression cassette

Expression

Bacterial

Mutation

A37U in tRNAfMet

Promoter

metY native promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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ptRNAfMet-A37G

Plasmid

#196658

Purpose

Plasmid for overexpression of mutated initiator tRNA (A37G)

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

tRNAfMet-A37G expression cassette

Expression

Bacterial

Mutation

A37G in tRNAfMet

Promoter

metY native promoter

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPRC6-ProC

Plasmid

#198430

Purpose

mkate2 fluorescent reporter gene under control of promoter ProC. Used as a control plasmid in reporter gene assays.

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Insert

mKate2

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProC and no promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPRC6-ProD

Plasmid

#198431

Purpose

mkate2 fluorescent reporter gene under control of promoter ProD. Used as a control plasmid in reporter gene assays.

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Insert

mKate2

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProD and no promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-N-term_F

Plasmid

#146300

Purpose

Mammalian Expression of HsTNRC6A-Nterm

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-Nterm (TNRC6A Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1-C-term_F

Plasmid

#146301

Purpose

Mammalian Expression of HsTNRC6Biso1-Cterm

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6Biso1-Cterm (TNRC6B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1-delMI_F

Plasmid

#146302

Purpose

Mammalian Expression of HsTNRC6Biso1-delMI

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6Biso1-delMI (TNRC6B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1-delMII_F

Plasmid

#146303

Purpose

Mammalian Expression of HsTNRC6Biso1-delMII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6Biso1-delMII (TNRC6B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1-delMI-MII_F

Plasmid

#146304

Purpose

Mammalian Expression of HsTNRC6Biso1-delMI-MII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6Biso1-delMI-MII (TNRC6B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1-N-term_F

Plasmid

#146305

Purpose

Mammalian Expression of HsTNRC6Biso1-Nterm

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6Biso1-Nterm (TNRC6B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C-delMI-MII-AGOhook_F

Plasmid

#146312

Purpose

Mammalian Expression of HsTNRC6C-delMI-MII-AGOhook

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-delMI-MII-AGOhook (TNRC6C Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of Hs…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-AP-1 σ1B

Plasmid

#198335

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-1 σ1B

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Expresses a LELQMNRRY nonapeptide after the last …

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-AP-1 σ1A

Plasmid

#198334

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-1 σ1A

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Expresses a LELQMNRRY nonapeptide after the last …

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ2

Plasmid

#198173

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-2 μ2

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0597

Plasmid

#196166

Purpose

MoClo Level 1 luciferase reporter: MinSyn_301 reporter [MinSyn_301::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_301 reporter [MinSyn_301::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0602

Plasmid

#196168

Purpose

MoClo Level 1 luciferase reporter: MinSyn_303 reporter [MinSyn_303::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_303 reporter [MinSyn_303::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0601

Plasmid

#196167

Purpose

MoClo Level 1 luciferase reporter: MinSyn_302 reporter [MinSyn_302::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_302 reporter [MinSyn_302::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0604

Plasmid

#196169

Purpose

MoClo Level 1 luciferase reporter: MinSyn_305 reporter [MinSyn_305::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_305 reporter [MinSyn_305::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0607

Plasmid

#196171

Purpose

MoClo Level 1 luciferase reporter: MinSyn_310 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_310 reporter [MinSyn_308::TMV::LucF::flag::ocsT]

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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