We narrowed to 11,848 results for: NSI
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Plasmid#197101PurposeTo express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanineDepositorInsertTyrRS variant, amber suppressor tRNA, kanamycin resistance gene
ExpressionBacterialMutationThr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA
Plasmid#154775Purposeplasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAA in GFP reporter, UUA anticodon and G10A, …PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Nkx2-1*/Neo
Plasmid#31272DepositorInsertNkx2-1* (Nkx2-1 Mouse)
UseRetroviralExpressionMammalianMutationA shNkx2-1 insensitive Nkx2-1 cDNA was created by…Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag
Plasmid#185682PurposeLow-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.DepositorAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA
Plasmid#154776Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation102TAG 150TAA in GFP reporter, hybrid PylT with A…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-N1-iChloC_CA
Plasmid#98171Purposeslow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct iChloC_C128A gene
TagsmCherryExpressionMammalianMutationE83Q, E90R, E101S, C128A, T159C, D156NPromoterCMV (+enhancer)Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRF.1 udsVenus (P_JUND)
Plasmid#58692PurposeA rapidly responsive reporter of endogenous JUND promoter activity (Lentiviral expression vector for reporter ultradestabilized Venus)DepositorAvailable SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 150TAG
Plasmid#154772Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAG in GFP reporter, hybrid PylT with A41AA an…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(T17N)
Plasmid#164902PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domainDepositorInsertRaichuEV-Rac1 (RAC1 Human)
ExpressionMammalianMutationT17N mutation in the Rac1 domain which make the s…Available SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAG
Plasmid#154777Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation102TAA 150TAG in GFP reporter, hybrid PylT with …PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag
Plasmid#185681PurposeLow-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Human, Synthetic)
TagsHaloTagExpressionMammalianMutationDeletion of lumenal domainAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag
Plasmid#185684PurposeLow-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.DepositorAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-hMSN
Plasmid#211823PurposeExpress human MSN in mammalian cellsDepositorAvailable SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pF713-pEGFP-N1-Hs-FL-METTL20 wt
Plasmid#85114Purposetransfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusionDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNL2119 pARF7 GG0-GG1
Plasmid#195910PurposeLevel 0 pARF7 promoter with GG0 and GG1 golden gate linkersDepositorInsertpARF7 (NPH4 Mustard Weed)
UseSynthetic BiologyAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc-oDam_f_GFPoNLS
Plasmid#85818PurposeiDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc oDam_f_GFPoNLS
TagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…Available SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOUPc-UL148-HA
Plasmid#179343PurposeAll-in-one "tet-on" 3G lentiviral vector plasmid encoding human cytomegalovirus UL148 ER resident glycoprotein with a C-terminal HA tag fused to its cytoplasmic tailDepositorInserthuman cytomegalovirus UL148 fused to HA epitope
UseLentiviral and Synthetic Biology; Hiv-1 based rep…TagsHA tagExpressionMammalianMutationinsert matches UL148 from strain TB40/EPromoterTet responsive element 3GAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only