We narrowed to 30,936 results for: LIS;

1. pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

   Plasmid

   #200641

   Purpose

   Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

   Depositor

   Fleur Ferguson, Justin English

   Article

   Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

   Insert

   miniTurboID-6xGGSG linker-FKBPF36V-2xHA
   

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCGN N-Ras wt

   Plasmid

   #14723

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

   Insert

   N-ras (NRAS Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30-HPF1-His-Sumo-Flag

   Plasmid

   #111577

   Purpose

   Expresses full length HPF1 in E. coli

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   HPF1 (HPF1 Human)
   

   Tags

   6xHis-Sumo-FLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLuc-N-HiBiT

   Plasmid

   #207117

   Purpose

   Bacterially expressed HiBiT-FLuc for Ni-NTA purification

   Depositor

   James Inglese

   Article

   Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

   Insert

   luciferin 4-monooxygenase
   

   Tags

   HiBiT Tag and His-tag with thrombin site

   Expression

   Bacterial

   Mutation

   Codon optmization for expression in BL21DE3

   Promoter

   T7 Promotor

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115267

   Purpose

   Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   LbuCas13a
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNAintron-CHIKV-Structural polyprotein

   Plasmid

   #215698

   Purpose

   Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV structural polyprotein Capsid-E3-E2-6K-E1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. GFP-BLM

   Plasmid

   #80070

   Purpose

   Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)

   Depositor

   Nathan Ellis

   Article

   Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98

   Insert

   BLM (BLM Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   If correct ID, the cDNA is from HeLa and has char…

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDuBir-Lbu-dCas13a-avitag

   Plasmid

   #100817

   Purpose

   Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a

   Depositor

   Mitchell O'Connell

   Article

   Tambe et al Cell Rep. 2018 Jul 24;24(4):1025-1036. d

   Insert

   Lbu_Cas13a_R472A_H477A_R1048A_H1053A
   

   Tags

   AviTag and His6-MBP

   Expression

   Bacterial

   Mutation

   R472A, H477A, R1048A, H1053A

   Promoter

   T7

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-Puro-REDD1-HA

   Plasmid

   #133550

   Purpose

   Expresses HA tagged REDD1 in Mammalian cells

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG sHRPa-FRB-pre mGRASP

    Plasmid

    #73145

    Purpose

    Large fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPa-FRB-pre mGRASP
    

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Biomolecular FeedBack (FB)

    Plasmid

    #109391

    Purpose

    This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pJ23100mut-dCas9-T- phtpG1-sgRNA
    

    Expression

    Bacterial

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNAintron-CDC50A-c3xFLAG

    Plasmid

    #215702

    Purpose

    Produces human CDC50A-c3xFLAG, a chaperone to phosphatidylserine flippase complexes

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CDC50A (TMEM30A Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNAintron-CHIKV-capsid protein

    Plasmid

    #215701

    Purpose

    Produces the Chikungunya virus capsid protein

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV capsid
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. HSC1-HS4-GiP

    Plasmid

    #58540

    Purpose

    Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Inserts

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Core HS4 insulator sequence (dimer)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    Human EF1-alpha

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. KTK_002

    Plasmid

    #180527

    Purpose

    Entry vector containing J23104 promoter

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    J23104 promoter
    

    Expression

    Bacterial

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLux01

    Plasmid

    #78281

    Purpose

    mRFP1 expressed behind an AHL-inducible construct

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    AHL inducible mRFP1
    

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCGN N-Ras 12D

    Plasmid

    #14725

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

    Insert

    N-ras (NRAS Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    12D

    Available Since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

19. AEB-No-aptamer-controlmRFP1

    Plasmid

    #63848

    Purpose

    This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.

    Depositor

    Howard Salis

    Article

    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

    Insert

    No-aptamer control mRNA
    

    Use

    Synthetic Biology

    Promoter

    J23100

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNAintron-CHIKV-nsp3-c3xFLAG

    Plasmid

    #215696

    Purpose

    Produces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp3
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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