Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 17,160 results for: REV

Showing: 11561 - 11580 of 17160 results

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pADH1-MTS-ABE8e-P2A-EGFP

Plasmid

#251973

Purpose

Yeast mtDNA editing with a mid-strength promoter for lower expression level

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

ABE8e

Use

CRISPR

Tags

MTS and V5-P2A-EGFP

Expression

Yeast

Promoter

ADH1

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pPT24-mtArg8m*-V5-UTR-HH-ntsgRNA-HDV

Plasmid

#251982

Purpose

For biolistic transformation to express Arg8m* and a non-targeting control sgRNA from mtDNA

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Inserts

mtArgm*
HH-nt sgRNA-HDV

Tags

V5

Expression

Yeast

Mutation

premature stop CAA->TAA, Q79*

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pVG286

Plasmid

#227756

Purpose

Expresses a version of light-inducible transcription factor EL222 (Gly80Glu) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Gly80Glu mutant

Promoter

PGK1pr

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-R56X

Plasmid

#248137

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with R56X mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-R56X (Vamp2 Rat)

Expression

Mammalian

Mutation

R56X

Promoter

Ubiquitin

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-V43del

Plasmid

#248139

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with V43del deletion

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-V43del (Vamp2 Rat)

Expression

Mammalian

Mutation

V43del

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-S75P

Plasmid

#248138

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with S75P mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-S75P (Vamp2 Rat)

Expression

Mammalian

Mutation

S75P

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-R56L

Plasmid

#248136

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with R56L mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-R56L (Vamp2 Rat)

Expression

Mammalian

Mutation

R56L

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-G73W

Plasmid

#248135

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with G73W mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-G73W (Vamp2 Rat)

Expression

Mammalian

Mutation

G73W

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-F77S

Plasmid

#248134

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with F77S mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-F77S (Vamp2 Rat)

Expression

Mammalian

Mutation

F77S

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-A67P

Plasmid

#248132

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with A67P mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-A67P (Vamp2 Rat)

Expression

Mammalian

Mutation

A67P

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-I45del

Plasmid

#248140

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with I45del deletion

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-I45del (Vamp2 Rat)

Expression

Mammalian

Mutation

I45del

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-E78A

Plasmid

#248133

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with E78A mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-E78A (Vamp2 Rat)

Expression

Mammalian

Mutation

E78A

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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VAMP3(S48A)-mCherry

Plasmid

#193635

Purpose

Expresses S48A phosphodead VAMP3 fused to mCherry

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

VAMP3 (Vamp3 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

changed serine 48 to alanine

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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PKA1284

Plasmid

#239367

Purpose

GST-Las17 (342-394) minimal component

Depositor

Kathryn Ayscough

Insert

Las17 (amino acids 342-392)

Tags

GST

Expression

Bacterial

Mutation

fragment corresponding to amino acids 342-392

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-NET1

Plasmid

#232893

Purpose

Plasmid expressing Cas9 and gRNA GTGCCACTAATGGATCCATG which targets the NET1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

NET1 gRNA (NET1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_DBDHA

Plasmid

#232987

Purpose

Galactose iduced expression of Gcn4 DBDAHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

DBD

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_DBD

Plasmid

#232985

Purpose

Galactose iduced expression of Gcn4 DBD in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 DBD

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_DBDGFP

Plasmid

#232999

Purpose

Galactose iduced expression of Gcn4 DBDGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

DBD

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 StoL

Plasmid

#231882

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 StoL

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 StoL

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S104L, S117L, S136L, S144L

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 E2K2

Plasmid

#231868

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 E2K2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E2K2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S104K, S117E, S136E, S144K

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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