We narrowed to 1,174 results for: Aspa

1. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

   Plasmid

   #129039

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA11 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

   Plasmid

   #129030

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA2 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

   Plasmid

   #129031

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA3 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

   Plasmid

   #129032

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA4 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMBP-parallel1-GK5_v2 (D443N)

   Plasmid

   #134293

   Purpose

   Expresses GK5 (isoform 2)-D443N mutant in bacteria

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk5 (Gk5 Mouse)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed Aspartic Acid 443 to Asparagine

   Promoter

   T7

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBP-parallel1-GK5_v2 (D280N)

   Plasmid

   #134290

   Purpose

   Expresses GK5 (isoform 2)-D280N mutant in bacteria

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk5 (Gk5 Mouse)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed Aspartic Acid 280 to Asparagine

   Promoter

   T7

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMBP-parallel1-GK5_v2 (D30N)

   Plasmid

   #134289

   Purpose

   Expresses GK5 (isoform 2)-D30N mutant in bacteria

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk5 (Gk5 Mouse)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed Aspartic Acid 30 to Asparagine

   Promoter

   T7

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETDuet_huCAD_ICADL (TevS)

   Plasmid

   #100098

   Purpose

   dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

   Depositor

   Jim Wells

   Article

   Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

   Insert

   DNA fragmentation factor subunit alpha (CAD, DFFA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two caspase cleavage sites (DETD-117 and DAVD-224…

   Promoter

   T7

   Available since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIG-FGF13A

   Plasmid

   #220793

   Purpose

   To express the human FGF13 isoform 1(FGF13A) in mammalian cells together with EGFP

   Depositor

   Pierre Vanderhaeghen

   Article

   Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

   Insert

   FGF13 (FGF13 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-ASNS-OE-IRES-Thy1.1

    Plasmid

    #192947

    Purpose

    Asparagine synthetase overexpression in murine cells

    Depositor

    Ping-Chih Ho

    Article

    Fernandez-Garcia et al Cell Rep. 2022 Nov 15;41(7):111639. doi:

    Insert

    Asparagine synthetase (Asns Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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