Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 24 results for: Aspa

Showing: 1 - 20 of 24 results

  1. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...activate a cascade of caspases, protease enzymes that cleave proteins. Executioner caspases are activated last... executioner caspase reporters. In these reporters, the FRET pair is linked by a caspase cleavage site...one of the linkers is a consensus caspase-3 (an executioner caspase) cleavage site (DEVD). Upon cleavage...used genetically encoded fluorescent executioner caspase reporters. Several apoptosis reporters have been...site. Cutting the linker by a caspase results in a change in fluorescence emission. FRET and fluorescence...Studying apoptosis in vivo using the FlipGFP-based caspase reporter The Shu Lab successfully used FlipGFP ...staurosporine, which induces apoptosis by activating caspase-3, and detected fluorescence within 2 to 5 hours...

  2. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...Solano S, Nguyen K , Lang E, Kaspar AA, Foust KD, Thomsen G, Fugere M, Kaspar BK (2019) Rett syndrome gene...collaboration between two gene therapy labs (Brian Kaspar and Steven Gray) and two MECP2 labs (Gail Mandel...development programs in biopharma. AveXis, co-founded by Kaspar, acquired by Novartis and rebranded to Novartis...

  3. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...D64V mutation, a single amino acid change (from aspartic acid to valine at position 64) which is sufficient...Ancliff, P., Webb, D. K., Schmidt, M., von Kalle, C., Gaspar, H. B., Thrasher, A. J. (2008). Insertional mutagenesis...

  4. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...directed to integrins that bind the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands. This... compared to those binding the Leucine-Valine-Aspartate-Proline (LVDP) motif. The Ha and Springer teams...

  5. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ... is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out...

  13. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...) designed and improved by the Loren Looger and Kaspar Podgorski Labs now has a new version: iGluSnFR4...

  15. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Montgomery, C.L., Hernandez, A., Chan, C.M. and Kaspar B.K. (2009). Intravascular AAV9 preferentially ...

  16. p53 Pathway

    Type
    Collection
    ...death agonist CASP3 Caspase 3, apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related...apoptosis-related cysteine peptidase CASP9 Caspase 9, apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3...

  17. Lentivirus Plasmids

    Type
    Collection
    ...viral titer Kasparov 22501 pHEF-VSVG N/A Envelope VSV-G-expressing envelope plasmid Kasparov 22500 pNHP...pNHP 2nd Packaging Contains gag, pol, tat, rev Kasparov 1732 pCPRDEnv 2nd Packaging part of the FELIX vector...

  18. Brain Initiative Collection

    Type
    Collection
    ...production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s Yellow ...production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2-dTom-nlsdTom AAV...

  19. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Methods. 2020 May 25. pii: 10.1038/s41592-020-0835-7. Kaspar Podgorski Calcium HaloCaMP1 chemigenetic calcium...synaptic transmission. Nat Methods. 2023 May 4. Kaspar Podgorski Glutamate FRET sensor to monitor glutamate... publication title or keywords (e.g. "RCaMP", "caspase", "mTORC1", "mitochondria"). ID Plasmid Description...

  20. Retrovirus Plasmids

    Type
    Collection
    ...pHEF-VSVG Envelope VSV-G-expressing envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common ...
Showing: 1 - 20 of 24 results