...activate a cascade of caspases, protease enzymes that cleave proteins. Executioner caspases are activated last... executioner caspase reporters. In these reporters, the FRET pair is linked by a caspase cleavage site...one of the linkers is a consensus caspase-3 (an executioner caspase) cleavage site (DEVD). Upon cleavage...used genetically encoded fluorescent executioner caspase reporters. Several apoptosis reporters have been...site. Cutting the linker by a caspase results in a change in fluorescence emission. FRET and fluorescence...Studying apoptosis in vivo using the FlipGFP-based caspase reporter The Shu Lab successfully used FlipGFP ...staurosporine, which induces apoptosis by activating caspase-3, and detected fluorescence within 2 to 5 hours...

...Solano S, Nguyen K , Lang E, Kaspar AA, Foust KD, Thomsen G, Fugere M, Kaspar BK (2019) Rett syndrome gene...collaboration between two gene therapy labs (Brian Kaspar and Steven Gray) and two MECP2 labs (Gail Mandel...development programs in biopharma. AveXis, co-founded by Kaspar, acquired by Novartis and rebranded to Novartis...

...directed to integrins that bind the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands. This... compared to those binding the Leucine-Valine-Aspartate-Proline (LVDP) motif. The Ha and Springer teams...

...D64V mutation, a single amino acid change (from aspartic acid to valine at position 64) which is sufficient...Ancliff, P., Webb, D. K., Schmidt, M., von Kalle, C., Gaspar, H. B., Thrasher, A. J. (2008). Insertional mutagenesis...

... is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out...

...Exosome-Associated AAV by Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development...

...SF-Venus-iGluSnFR (itself an improvement over iGluSnFR), Kaspar Podgorski’s lab have recently made iGluSnFR3 variants...

...Aggarwal is a postbaccalaureate research technician in Kaspar Podgorski’s lab at Janelia Research Campus, HHMI...

...consequences of that change. For example, “Mutated aspartate 123 to Lysine (D123K), lowers biosensor affinity...

...research and science communication.     References 1. Gaspar, H. B., S. Howe, and A. J. Thrasher. "Gene therapy...

...glutamate sensor iGluSnFR from Loren Looger’s lab, Kaspar Podgorski and colleagues have now released iGluSnFR3...

...interest is mutated to a negatively charged residue, aspartate or glutamate, imparting the negative charge associated...

...consequences of that change. For example, "Mutated Aspartate 123 to Lysine (D123K), lowers biosensor affinity...

...Montgomery, C.L., Hernandez, A., Chan, C.M. and Kaspar B.K. (2009). Intravascular AAV9 preferentially ...

...death agonist CASP3 Caspase 3, apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related...apoptosis-related cysteine peptidase CASP9 Caspase 9, apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3...

...viral titer Kasparov 22501 pHEF-VSVG N/A Envelope VSV-G-expressing envelope plasmid Kasparov 22500 pNHP...pNHP 2nd Packaging Contains gag, pol, tat, rev Kasparov 1732 pCPRDEnv 2nd Packaging part of the FELIX vector...

...production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s Yellow ...production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2-dTom-nlsdTom AAV...

...Methods. 2020 May 25. pii: 10.1038/s41592-020-0835-7. Kaspar Podgorski Calcium HaloCaMP1 chemigenetic calcium...synaptic transmission. Nat Methods. 2023 May 4. Kaspar Podgorski Glutamate FRET sensor to monitor glutamate... publication title or keywords (e.g. "RCaMP", "caspase", "Dynamic Visualization of mTORC1 Activity in ...

...pHEF-VSVG Envelope VSV-G-expressing envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common ...

...Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression pnCasPA-BEC - A cytidine deaminase-mediated base-editing...