Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 15 results for: Aspa

Showing: 1 - 15 of 15 results

  1. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...activate a cascade of caspases, protease enzymes that cleave proteins. Executioner caspases are activated last... executioner caspase reporters. In these reporters, the FRET pair is linked by a caspase cleavage site...one of the linkers is a consensus caspase-3 (an executioner caspase) cleavage site (DEVD). Upon cleavage...used genetically encoded fluorescent executioner caspase reporters. Several apoptosis reporters have been...site. Cutting the linker by a caspase results in a change in fluorescence emission. FRET and fluorescence...Studying apoptosis in vivo using the FlipGFP-based caspase reporter The Shu Lab successfully used FlipGFP ...staurosporine, which induces apoptosis by activating caspase-3, and detected fluorescence within 2 to 5 hours...

  2. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...Solano S, Nguyen K , Lang E, Kaspar AA, Foust KD, Thomsen G, Fugere M, Kaspar BK (2019) Rett syndrome gene...collaboration between two gene therapy labs (Brian Kaspar and Steven Gray) and two MECP2 labs (Gail Mandel...development programs in biopharma. AveXis, co-founded by Kaspar, acquired by Novartis and rebranded to Novartis...

  3. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...D64V mutation, a single amino acid change (from aspartic acid to valine at position 64) which is sufficient...Ancliff, P., Webb, D. K., Schmidt, M., von Kalle, C., Gaspar, H. B., Thrasher, A. J. (2008). Insertional mutagenesis...

  4. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...directed to integrins that bind the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands. This... compared to those binding the Leucine-Valine-Aspartate-Proline (LVDP) motif. The Ha and Springer teams...

  5. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ... is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out...

  11. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...glutamate sensor iGluSnFR from Loren Looger’s lab, Kaspar Podgorski and colleagues have now released iGluSnFR3...

  13. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...) designed and improved by the Loren Looger and Kaspar Podgorski Labs now has a new version: iGluSnFR4...

  14. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...consequences of that change. For example, "Mutated Aspartate 123 to Lysine (D123K), lowers biosensor affinity...

  15. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...Montgomery, C.L., Hernandez, A., Chan, C.M. and Kaspar B.K. (2009). Intravascular AAV9 preferentially ...
Showing: 1 - 15 of 15 results