We narrowed to 1,606 results for: Sp1

1. pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)

   Plasmid

   #160540

   Purpose

   Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.

   Depositor

   Kenneth Johnson

   Article

   Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

   Inserts

   SARS-CoV-2 NSP7 (ORF1ab Synthetic)
   SARS-CoV-2 NSP8 (ORF1ab Synthetic)
   SARS-CoV-2 NSP12 (ORF1ab Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

   Plasmid

   #193048

   Purpose

   Heatshock expressed Cas9

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   hsp16.41p

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a 6xHis PreScission SARS-CoV-2 nsp13

   Plasmid

   #159390

   Purpose

   Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

   Depositor

   Tarun Kapoor

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5954 pcDNA-Xpress-His-USP15 C269A

   Plasmid

   #23217

   Depositor

   Peter Howley

   Article

   Vos et al J Virol. 2009 Sep . 83(17):8885-92.

   Insert

   USP15 (USP15 Human)
   

   Use

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   C269A

   Promoter

   Available since

   April 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiUBC CASP1[CARD]-mSG2 (inflammasome reporter)

   Plasmid

   #228044

   Purpose

   Lentiviral stable Inflammasome speck reporter, designed from PMC9623368.

   Depositor

   Wade Harper

   Insert

   CASP1[CARD]-mSG2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223 CASP1[CARD]-mSG2 (inflammasome reporter)

   Plasmid

   #228043

   Purpose

   DONR vector for inflammasome speck reporter, designed from PMC9623368.

   Depositor

   Wade Harper

   Insert

   CASP1[CARD]-mSG2
   

   Use

   Gateway donr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

   Plasmid

   #176060

   Purpose

   Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K164A and H165A

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

   Plasmid

   #176059

   Purpose

   Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R124A and K125A

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

   Plasmid

   #175421

   Purpose

   Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 nsp1
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX C_K nsp1 K164A/H165A CoV2

    Plasmid

    #175517

    Purpose

    Expresses C_K for bioconjugation on nsp1

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    C_K nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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