We narrowed to 1,150 results for: maz

1. pEVOL-NBK-1

   Plasmid

   #207639

   Purpose

   A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The evolved synthetase NBK-1 contains mutations Y…

   Promoter

   araBAD

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILGFPEasy9R

   Plasmid

   #218291

   Purpose

   Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS306-URA3-PHIS3-AqLumSynth –Sapphire

   Plasmid

   #116931

   Purpose

   Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   lumazine synthase
   

   Use

   Tags

   Sapphire

   Expression

   Yeast

   Mutation

   Promoter

   HIS3

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF1898

   Plasmid

   #144116

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PATZ1 (PATZ1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. WP_010880027.1

   Plasmid

   #194729

   Purpose

   Lumazine synthase of the hyperthermophile Aquifex aeolicus bacterium

   Depositor

   Fang Li

   Article

   Geng et al PLoS Pathog. 2021 Sep 7;17(9):e1009897.

   Insert

   nanoparticle-proteinA and His tag
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF1900

   Plasmid

   #144117

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PATZ1 (PATZ1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF3181

   Plasmid

   #144657

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PATZ1 (PATZ1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

   Plasmid

   #140020

   Purpose

   Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

   Promoter

   EF1

   Available since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pD649-HAsp-SEMA6B-COMP5AP-AviTag-9xHis

   Plasmid

   #157482

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA6B (SEMA6B Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD649-HAsp-SEMA6B-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156918

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA6B (SEMA6B Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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