We narrowed to 21,719 results for: ESC;

1. pUPD2 RDF ( GB1498)

   Plasmid

   #160579

   Purpose

   Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   RDF
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CDK2-3XFlag-pBabe

   Plasmid

   #73557

   Purpose

   vector encoding a fusion protein between CDK2 and 3 Flag tags

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   CDK2 (CDK2 Human)
   

   Use

   Retroviral

   Tags

   3X-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pNCST-AvicFP2

   Plasmid

   #129505

   Purpose

   Expresses AvicFP2 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNCST-AausFP2

   Plasmid

   #129500

   Purpose

   Expresses AausFP2 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPICZ-aC-KHC-BZ

   Plasmid

   #160427

   Purpose

   Expression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)

   Depositor

   Todd Oakley

   Article

   Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

   Insert

   luciferase
   

   Use

   Luciferase

   Tags

   6x Histidine tag, Yeast alpha secretion factor, c…

   Expression

   Yeast

   Mutation

   Promoter

   AOX

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40

   Plasmid

   #98931

   Purpose

   Cre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   iGluSnFr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Clover-TOMM20-N-10

   Plasmid

   #56307

   Purpose

   Localization: Outer Mitochondrial Membrane, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   TOMM20 (TOMM20 Human)
   

   Use

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. mRuby2-TOMM20-N-10

   Plasmid

   #55913

   Purpose

   Localization: Outer Mitochondrial Membrane, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   TOMM20 (TOMM20 Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pRM27

   Plasmid

   #163695

   Purpose

   ubb promoter driven expression of lck:mNeonGreen

   Depositor

   David Matus

   Article

   Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

   Insert

   lck:mNeonGreen (lck Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   ubb

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA6-N-3XFLAG-Ubac2

    Plasmid

    #123597

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    ubiquitin associated domain containing 2 (Ubac2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. eNS2B47NS3 L30SF31S

    Plasmid

    #155319

    Purpose

    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutant

    Depositor

    Dahai Luo

    Article

    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

    Insert

    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    L30 to S, F31 to S

    Promoter

    M13

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p20StAD

    Plasmid

    #190268

    Purpose

    Constitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.

    Depositor

    Assaf Mosquna

    Article

    Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2

    Type

    Empty backbone

    Use

    Synthetic Biology; Yeast genome-integrating vector

    Tags

    GAL4-AD

    Expression

    Mutation

    Promoter

    TDH3

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CAG-miRFP2

    Plasmid

    #178968

    Purpose

    Expresses miRFP2 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    miRFP2
    

    Use

    AAV

    Tags

    no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CAG

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mPapaya1-Farnesyl-5

    Plasmid

    #56650

    Purpose

    Localization: Membrane, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mPapaya1

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mRuby2-Actin-C-18

    Plasmid

    #55889

    Purpose

    Localization: Actin, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. WT N-His Ana GvpC in pET28a

    Plasmid

    #153294

    Purpose

    Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (N-terminal his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added a hexahistidine tag after the methionine at…

    Promoter

    T7

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mOrange2-Fibrillarin-7

    Plasmid

    #57957

    Purpose

    Localization: Nucleus/Nucleoli, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pLVX-mCherry-SPASTIN, F124D

    Plasmid

    #180648

    Purpose

    Lentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Starts at M87, F124D mutation, has siRNA resistan…

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUPD2 PhiC31 integrase (GB1496)

    Plasmid

    #160578

    Purpose

    Streptomyces phage PhiC31 integrase, complete CDS

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRD408

    Plasmid

    #178199

    Purpose

    Expression of H-NS from its natural promoter

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    phns + hns (hns Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    hns promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge