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pML107-SAM50

Plasmid

#232895

Purpose

Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

SAM50 gRNA (SAM50 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA355_sgCiPOLR2D

Plasmid

#229022

Purpose

Expression of a CRISPRi doxycycline inducible guide targeting POLR2D

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

POLR2D gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA052H_sgCh2

Plasmid

#229023

Purpose

Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

sgChr2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA052H_sgFOCAD #2

Plasmid

#229024

Purpose

Expression of a EnAsCas12a guide targeting FOCAD

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

FOCAD gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_051d_sgCiChr2-2

Plasmid

#228940

Purpose

Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

sgChr2-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-HRAS(GV12)

Plasmid

#233181

Purpose

Retroviral expression of HRAS(G12V)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

HRAS(G12V) (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-Isl1-P2A-mRuby2

Plasmid

#233169

Purpose

Retroviral expression of Isl1 with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-P2A-mRuby2 (Isl1 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mLhx3-P2A-mRuby2

Plasmid

#233175

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-mNgn2x3HA-P2A-mRuby2

Plasmid

#233157

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Mouse, Synthetic)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-Isl1-P2A-mRuby2

Plasmid

#233158

Purpose

Retroviral expression of Isl1 with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-P2A-mRuby2 (Isl1 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-mLhx3-P2A-mRuby2

Plasmid

#233159

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mNgn2x3HA-P2A-mRuby2

Plasmid

#233163

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Mouse, Synthetic)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-ARB1

Plasmid

#232888

Purpose

Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

ARB1 gRNA (ARB1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-RER2

Plasmid

#232884

Purpose

Plasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

RER2 gRNA (RER2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-LEU2-3

Plasmid

#232894

Purpose

Plasmid expressing Cas9 and gRNA GTTCTTAACTAGGATCATGG which targets the RER2 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

LEU2 gRNA (RER2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRS313_MSN5-LY00018

Plasmid

#232903

Purpose

Plasmid carrying MSN5 from LY00018 (YJM975) with its native promoter and terminator.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

MSN5 (MSN5 Budding Yeast)

Expression

Yeast

Mutation

G1563A

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-HygMx4-URA3-3

Plasmid

#232886

Purpose

Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

URA3 gRNA (URA3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-HygMx4-URA3-2

Plasmid

#232885

Purpose

Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

URA3 gRNA (URA3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Puro-DEST (JDW 925)

Plasmid

#229808

Purpose

A puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning system

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-AVO1

Plasmid

#232889

Purpose

Plasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

AVO1 gRNA (AVO1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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